Cynomolgus Monkey B7-2/CD86 Antibody Summary
Leu20-His239
Accession # XP_005548057
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Western Blot Shows Human B7‑2/CD86 Specificity by Using Knockout Cell Line. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and human B7-2/CD86 knockout Ramos cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Cynomolgus Monkey B7‑2/CD86 Monoclonal Antibody (Catalog # MAB10601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for B7‑2/CD86 at approximately 70 kDa (as indicated) in the parental cell line, but is not detectable in knockout cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Detection of Human B7‑2/CD86 by Western Blot. Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Cynomolgus Monkey B7‑2/CD86 Monoclonal Antibody (Catalog # MAB10601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for B7‑2/CD86 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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B7‑2/CD86 in Human Tonsil. B7‑2/CD86 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Cynomolgus Monkey B7‑2/CD86 Monoclonal Antibody (Catalog # MAB10601) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-2/CD86
B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance (1, 2). Within the ECD, cynomolgus B7-2 shares 96%, 57%, and 56% amino acid sequence identity with human, mouse and rat B7-2, respectively. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes (2-5), as well as on vascular endothelial cells (6). B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co-stimulation (3, 5, 7-9). B7-1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up‑regulated and recruited to the immunological synapse (IS) at the onset of T cell activation (3, 5, 7, 8). CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function (10). B7-2 is expressed earlier than B7-1 following APC activation (4), and both proteins bind with higher affinity to CTLA-4 than to CD28 (8). B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS (11). The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response (12). Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses (13).
- Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515.
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
- Lenschow, D.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11054.
- Hathcock, K.S. et al. (1993) Science 262:905.
- Seino, K. et al. (1995) Int. Immunol.7:1331.
- Chen, C. et al. (1994) J. Immunol. 152:4929.
- Lanier, L.L. et al. (1995) J. Immunol. 154:97.
- Rudd, C.E. et al. (2009) Immunol. Rev. 229:12.
- Wing, K. et al. (2011) Trends Immunol. 32:428.
- Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
- Kuchroo, V.K. et al. (1995) Cell 80:707.
- Short, J.J. et al. (2006) Virus Res. 122:144.
Product Datasheets
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