DAPI
Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity: ≥95%
Biological Activity
DAPI is a fluorescent DNA stain / dye. DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. DAPI stain can be used in flow cytometry, DNA visualization and quantification in histochemistry/biochemistry, to assess apoptosis, and to visualize mouse embryos or fetal organs. DAPI stain can be used in fixed cells and is cell-permeable at high concentrations providing effective cell staining. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Em/Ex λ = 350/470 nm respectively.Scientific Data
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DAPI staining. HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.
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DAPI counterstaining alongside MitoBrilliant™ Live 646. HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.
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RNAScope™ Assay with DAPI Counterstain. 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).
Optical Data for DAPI
| Emission Color | Blue |
|---|---|
| λabs | 350 nm |
| λem | 470 nm |
| Application | Flow cytometry, ISH |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.
Russell et al.
Nature, 1975;253:461 -
Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen.
Mildner et al.
Cancer Lett., 1978;4:89
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Citations for DAPI
The citations listed below are publications that use Tocris products. Selected citations for DAPI include:
14 Citations: Showing 1 - 10
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Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cells.
Authors: Thanaphum Et al.
J Dent Sci 2023;18:105-111
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Gut Microbiota-Derived Glutamine Attenuates Liver Ischemia/Reperfusion Injury via Macrophage Metabolic Reprogramming.
Authors: Yu Et al.
Cell Mol Gastroenterol Hepatol 2023;15:1255-1275
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BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity.
Authors: Yelyzaveta Et al.
Nat Commun 2023;14:1085
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Regulation of Cx43 Gap Junction Intercellular Communication by Bruton's Tyrosine Kinase and Interleukin-2-Inducible T-Cell Kinase.
Authors: Paul L Et al.
Biomolecules 2023;13
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Erythropoietin administration exerted neuroprotective effects against cardiac ischemia/reperfusion injury.
Authors: Nipon Et al.
Curr Res Pharmacol Drug Discov 2022;3:100124
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A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.
Authors: Marit H Et al.
Cell Rep 2022;39:110663
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Modulation of mitochondrial dynamics rescues cognitive function in rats with 'doxorubicin-induced chemobrain' via mitigation of mitochondrial dysfunction and neuroinflammation.
Authors: Titikorn Et al.
FEBS J 2022;289:6435-6455
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Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance.
Authors: Satu Et al.
J Exp Med 2022;219
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HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.
Authors: Digvijay Et al.
Science 2021;371
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A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.
Authors: Elizabeth Et al.
STAR Protoc 2021;2:100261
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Granzyme A Produced by γ9δ2 T Cells Activates ER Stress Responses and ATP Production, and Protects Against Intracellular Mycobacterial Replication Independent of Enzymatic Activity.
Authors: Daniel F Et al.
Front Immunol 2021;12:712678
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Gut-Resident Lactobacilli Activate Hepatic Nrf2 and Protect Against Oxidative Liver Injury.
Authors: Dean P Et al.
Cell Metab 2020;31:956-968.e5
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Indirect regulation of HMGB1 release by gasdermin D.
Authors: Allen Et al.
Nat Commun 2020;11:4561
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NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells.
Authors: Thanaphum Et al.
Sci Rep 2020;10:13329
FAQs
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