E 64
Chemical Name: (2S,3S)-3-[[[(1S)-1-[[[4-(Aminoiminomethyl)amino]butyl]amino]carbonyl]-3-methylbutyl]amino]carbonyl]-2-oxiranecarboxylic acid
Purity: ≥98%
Biological Activity
E 64 is a potent and irreversible cysteine protease inhibitor (IC50 values are 1.4, 2.5 and 4.1 nM for cathepsin K, L and S respectively. Also inhibits papain, calpain, cathepsin B and cathepsin H. Has no effect on serine proteases. Reduces HSC-3 tongue cancer cell invasion and induces oxidative stress and apoptosis in filarial parasites. Also inhibits autophagy-associated lysosomal degradation in vitro.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay.
Susa et al.
J.Transl.Med., 2004;2:6 -
Cathepsin K is present in invasive oral tongue squamous cell carcinoma in vivo and in vitro.
Bitu et al.
PLoS ONE, 2013;8:e70925 -
Inhibition of cathepsin B by E-64 induces oxidative stress and apoptosis in filarial parasite.
Wadhawan et al.
PLoS ONE, 2014;9:e93161 -
Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity.
Zhao et al.
Nat.Cell.Biol., 2010;12:665
Product Datasheets
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Citations for E 64
The citations listed below are publications that use Tocris products. Selected citations for E 64 include:
4 Citations: Showing 1 - 4
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Microglial P2X4 receptors promote ApoE degradation and contribute to memory deficits in Alzheimer's disease.
Authors: Bénédicte Et al.
Cell Mol Life Sci 2023;80:138
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A recombinant bovine adenoviral mucosal vaccine expressing mycobacterial antigen-85B generates robust protection against tuberculosis in mice.
Authors: Min Et al.
Cell Rep Med 2021;2:100372
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Apolipoprotein E is a concentration-dependent pulmonary danger signal that activates the NLRP3 inflammasome and IL-1β secretion by bronchoalveolar fluid macrophages from asthmatic subjects.
Authors: Xianglan Et al.
J Allergy Clin Immunol 2019;144:426-441.e3
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Tricyclic Antidepressants amitript. and desipr. Induced Neurotoxicity Associated with Parkinson's Disease.
Authors: Lee Et al.
PLoS One 2015;38:734
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