ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free
ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free Summary
Animal Component-Free Media for the ex vivo expansion and culture of human Natural Killer lymphocytes.Key Benefits
• Supports feeder- and serum-free expansion of NK cells
• Phenol red-free formulation
• Animal component-free media to support patient safety
• Supports NK cell expansion from PBMCs or purified CD3+-depleted PBMCs
• Protocol to support feeder-cell G-Rex culture process
• Protocol to support feeder-free G-Rex culture process
Why Use ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free?
Natural Killer (NK) cells play an important role in both the adaptive and innate immune responses that control infection, autoimmunity, and tumor immunosurveillance. Due to their intrinsic and non-specific anti-tumor activity, human NK cells have been employed as anti-cancer therapies. Robust expansion and maintenance of NK Cells is critical to facilitate NK cell therapy.
Versatility of ExCellerate Human NK Cell Expansion Media
ExCellerate Human NK Cell Expansion Media is optimized for NK cell expansion when used in combination with Recombinant Human Cytokines from R&D Systems; however, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-15, IL-12, IL-18, and IL-21) and cell activation methods, including particle, bead-based, or plate-bound Human NKp46/NCR1 Antibody. The media supports both feeder-based and feeder-free expansion methods.
Kit Contents
1 L of ExCellerate Human NK Cell Expansion Media, Animal Component-Free
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Scientific Data
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NK Cell Fold Expansion. Purified NK Cells from PBMCs were grown with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, 5% HuAB Serum, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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NK Cell Viability. Purified NK Cells from PBMCs were grown with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, 5% HuAB Serum, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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NK Cell Killing Capacity. Purified NK Cells from PBMCs were grown with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, 5% HuAB Serum, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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Phenotype analysis of 4 donors. Purified NK Cells from PBMCs were grown with ExCellerate™ NK Cell Expansion Media, Animal-Free (Cat # CCM037), irradiated feeder cells, 5% HuAB Serum, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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Serum-Free NK Cell Fold Expansion. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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Serum-Free Process NK Cell Viability. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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Serum-Free Process Killing Capacity. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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Serum-Free Process Phenotype. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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Expanded Phenotype Analysis on Serum-Free Process. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
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