FlowTACS Flow Cytometry Apoptosis Detection Kit

Catalog # Availability Size / Price Qty
4817-60-K
FlowTACS Flow Cytometry Apoptosis Detection Kit_4817-60-K
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FlowTACS Flow Cytometry Apoptosis Detection Kit Summary

FlowTACS® in situ - Fluorescein

A complete kit supplied with Streptavidin-Fluorescein and all the agents necessary for generating positive controls.

Label: Biotin/Streptavidin-Fluorescein
Testing Format: Flow Cytometry; Fluorescence Microscopy
Sample Type: Suspended or monolayer cells
Size: 60 Tests
Analysis of DNA Fragmentation in Cycloheximide- or Dexamethasone-treated Murine Thymocytes
Murine thymocytes were treated with 10 µg/mL cycloheximide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled according to the FlowTACS protocol (Catalog # 4817-60-K), and analyzed by flow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
The product  requires storage at both -20 to -70 °C and 2 - 8 °C.  Consult the product insert for specific storage temperatures. Do not use past expiration date.

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Scientific Data

Flow Cytometry FlowTACS Flow Cytometry Apoptosis Detection Kit_4817-60-K View Larger

Analysis of DNA Fragmentation in Cycloheximide- or Dexamethasone-treated Murine Thymocytes. Murine thymocytes were treated with 10 µg/mL cycloheximide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled according to the FlowTACS protocol (Catalog # 4817-60-K), and analyzed by flow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.

Citations for FlowTACS Flow Cytometry Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Global 5mC and 5hmC DNA Levels in Human Sperm Subpopulations with Differentially Protaminated Chromatin in Normo- and Oligoasthenozoospermic Males
    Authors: M Olszewska, O Kordyl, M Kamieniczn, M Fraczek, P J?drzejcza, M Kurpisz
    International Journal of Molecular Sciences, 2022-04-19;23(9):.  2022-04-19
  2. Cytoprotective Activity of Polyamines Is Associated with the Alternative Splicing of RAD51A Pre-mRNA in Normal Human CD4+ T Lymphocytes
    Authors: YA Gladilina, L Bey, A Hilal, EV Neborak, VG Blinova, DD Zhdanov
    International Journal of Molecular Sciences, 2022-02-07;23(3):.  2022-02-07
  3. Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia
    Authors: P Ayala-Ramí, C Machuca-Ac, T Gámez, S Quijano, A Barreto, JL Silva, M Olaya-C, R García-Rob
    Frontiers in Physiology, 2021-07-23;12(0):708824.  2021-07-23
  4. CircTM7SF3 contributes to oxidized low-density lipoprotein-induced apoptosis, inflammation and oxidative stress through targeting miR-206/ASPH axis in atherosclerosis cell model in vitro
    Authors: X Wang, M Bai
    BMC cardiovascular disorders, 2021-02-02;21(1):51.  2021-02-02
  5. Targeting IL-17A/glucocorticoid synergy to CSF3 expression in neutrophilic airway diseases
    Authors: S Ouyang, C Liu, J Xiao, X Chen, AC Lui, X Li
    JCI Insight, 2020-02-13;5(3):.  2020-02-13
  6. Insight into the mechanism of action of temporin-SHa, a new broad-spectrum antiparasitic and antibacterial agent.
    Authors: Raja Z, Andre S, Abbassi F, Humblot V, Lequin O, Bouceba T, Correia I, Casale S, Foulon T, Sereno D, Oury B, Ladram A
    PLoS ONE, 2017-03-20;12(3):e0174024.  2017-03-20
  7. Platelet-derived growth factor-C (PDGF-C) induces anti-apoptotic effects on macrophages through Akt and Bad phosphorylation.
    Authors: Son D, Na Y, Hwang E, Seok S
    J Biol Chem, 2014-01-13;289(9):6225-35.  2014-01-13
  8. The TACI receptor regulates T-cell-independent marginal zone B cell responses through innate activation-induced cell death.
    Authors: Figgett W, Fairfax K, Vincent F, Le Page M, Katik I, Deliyanti D, Quah P, Verma P, Grumont R, Gerondakis S, Hertzog P, O'Reilly L, Strasser A, Mackay F
    Immunity, 2013-09-05;39(3):573-83.  2013-09-05
  9. Division-linked generation of death-intermediates regulates the numerical stability of memory CD8 T cells.
    Authors: Nolz J, Rai D, Badovinac V, Harty J
    Proc Natl Acad Sci U S A, 2012-04-02;109(16):6199-204.  2012-04-02
  10. Theiler's virus-induced intrinsic apoptosis in M1-D macrophages is Bax mediated and restricts virus infectivity: a mechanism for persistence of a cytolytic virus.
    Authors: Son KN, Becker RP, Kallio P, Lipton HL
    J. Virol., 2008-02-20;82(9):4502-10.  2008-02-20
  11. Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling.
    Authors: Wang L, Schulz TC, Sherrer ES, Dauphin DS, Shin S, Nelson AM, Ware CB, Zhan M, Song CZ, Chen X, Brimble SN, McLean A, Galeano MJ, Uhl EW, D'Amour KA, Chesnut JD, Rao MS, Blau CA, Robins AJ
    Blood, 2007-08-29;110(12):4111-9.  2007-08-29
  12. Butyrate-induced apoptosis and cell cycle arrest in bovine kidney epithelial cells: involvement of caspase and proteasome pathways.
    Authors: Li CJ, Elsasser TH
    J. Anim. Sci., 2005-01-01;83(1):89-97.  2005-01-01

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