Goat IgG PE-conjugated Antibody Summary
Applications
Negative control for use in conjunction with R&D Systems antibodies for flow cytometry applications. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
This reagent has not been tested for immunohistochemistry.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Goat IgG Control by Flow Cytometry bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse VEGF R3/Flt-4 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB743P, filled histogram) or Goat IgG PE-conjugated Isotype Control Antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Goat IgG PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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FZ-AD005, A Novel DLL3-Targeted Antibody-drug Conjugate with Topoisomerase I Inhibitor, Shows Potent Antitumor Activity in Preclinical Models
Authors: Guo, Q;Gao, B;Song, R;Li, W;Xie, Q;Lou, S;Shen, J;Zhao, T;Zhang, Y;Wu, J;Yang, T;
Molecular cancer therapeutics
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Circulating tumor cells help differentiate benign ovarian lesions from cancer before surgery: A literature review and proof of concept study using flow cytometry with fluorescence imaging
Authors: Kuo, YC;Chuang, CH;Kuo, HC;Lin, CT;Chao, A;Huang, HJ;Wang, HM;Hsieh, JC;Chou, HH;
Oncology letters
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control -
Inhibition of interleukin-1beta reduces myelofibrosis and osteosclerosis in mice with JAK2-V617F driven myeloproliferative neoplasm
Authors: S Rai, E Grockowiak, N Hansen, D Luque Paz, CB Stoll, H Hao-Shen, G Mild-Schne, S Dirnhofer, CJ Farady, S Méndez-Fer, RC Skoda
Nature Communications, 2022-09-13;13(1):5346.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TCF-1 controls Treg cell functions that regulate inflammation, CD8+ T cell cytotoxicity and severity of colon cancer
Authors: A Osman, B Yan, Y Li, KD Pavelko, J Quandt, A Saadalla, MP Singh, M Kazemian, F Gounari, K Khazaie
Nature Immunology, 2021-08-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
JAK1 Inhibition Blocks Lethal Immune Hypersensitivity in a Mouse Model of Down Syndrome
Authors: KD Tuttle, KA Waugh, P Araya, R Minter, DJ Orlicky, M Ludwig, Z Andrysik, MA Burchill, BAJ Tamburini, C Sempeck, K Smith, R Granrath, D Tracy, J Baxter, JM Espinosa, KD Sullivan
Cell Rep, 2020-11-17;33(7):108407.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Radiation Triggers a Dynamic Sequence of Transient Microglial Alterations in Juvenile Brain
Authors: AM Osman, Y Sun, TC Burns, L He, N Kee, N Oliva-Vila, A Alevyzaki, K Zhou, L Louhivuori, P Uhlén, E Hedlund, C Betsholtz, VM Lauschke, J Kele, K Blomgren
Cell Rep, 2020-06-02;31(9):107699.
Species: Mouse
Sample Types:
Applications: IHC -
Mycobacterium tuberculosis Inhibits Autocrine Type I IFN Signaling to Increase Intracellular Survival
Authors: DA Banks, SE Ahlbrand, VK Hughitt, S Shah, KD Mayer-Barb, SN Vogel, NM El-Sayed, V Briken
J. Immunol., 2019-03-04;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulation of radiosensitivity by 4-methylumbelliferone via the suppression of interleukin-1 in fibrosarcoma cells
Authors: R Saga, K Hasegawa, K Murata, M Chiba, T Nakamura, K Okumura, E Tsuruga, Y Hosokawa
Oncol Lett, 2019-01-29;17(3):3555-3561.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Elevated expression of Tie1 is accompanied by acquisition of cancer stemness properties in colorectal cancer
Authors: M Torigata, D Yamakawa, N Takakura
Cancer Med, 2017-05-02;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy
Sci Rep, 2016-11-03;6(0):36012.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3).
Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J
J Biol Chem, 2015-08-25;290(41):24747-59.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Trafficking of LAG-3 to the surface on activated T cells via its cytoplasmic domain and protein kinase C signaling.
Authors: Bae J, Lee S, Park C, Lee Y, Chun T
J Immunol, 2014-08-08;193(6):3101-12.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
Br J Cancer, 2014-05-22;110(12):2865-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype.
Authors: Ylostalo J, Bartosh T, Coble K, Prockop D
Stem Cells, 2012-10-01;30(10):2283-96.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Neurotrophins regulate bone marrow stromal cell IL-6 expression through the MAPK pathway.
Authors: Rezaee F, Rellick SL, Piedimonte G
PLoS ONE, 2010-03-15;5(3):e9690.
Species: Human
Sample Types: Whole Cells
Applications: Control -
CD4(+)CD25(+) T regulatory cells inhibit CD8(+) IFN-gamma production during acute and chronic FIV infection utilizing a membrane TGF-beta-dependent mechanism.
Authors: Fogle JE, Mexas AM, Tompkins WA, Tompkins MB
AIDS Res. Hum. Retroviruses, 2010-02-01;26(2):201-16.
Species: Feline
Sample Types: Whole Cells
Applications: Control
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As a control of Mouse TGF-beta RII PE-conjugated Antibody (Catlog# FAB532P-025UG), it worked well.
