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Hoechst Janelia Fluor® 526

Catalog #: 7313 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7313/100U
Hoechst Janelia Fluor® 526 | Fluorescent DNA Stains
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Description: Fluorogenic, green-emitting DNA probe
Alternative Names: JF526-HST

Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate tetratrifluoroacetate

Purity: ≥95%

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Biological Activity

Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield = 0.04.

Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 is suitable for multicolor microscopy experiments and for use in live cell imaging.

Excitation maximum = 531 nm; emission maximum = 549 nm.

To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.

Optical Data for Hoechst Janelia Fluor® 526

Hoechst Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Green
λabs 531 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.126
Application Confocal, Super-resolution microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
1641.27
Formula:
C62H58F6N10O8.4CF3CO2H
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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Reconstitution Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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