Human 5-HT2B Antibody

Catalog #: MAB10322 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10322-100
MAB10322-SP

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Human 5-HT2B Antibody Summary

Species Reactivity

Human

Specificity

Detects human 5-HTR2B in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1014838

Purification

Protein A or G purified from ascites

Immunogen

Synthetic peptide containing human 5- HTR2B
Accession # P41595

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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5-HT2B in Mouse Cerebellum. 5-HT2B was detected in immersion fixed paraffin-embedded sections of mouse cerebellum using Mouse Anti-Human 5-HT2B Monoclonal Antibody (Catalog # MAB10322) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Purkinje neurons.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT2B

HTR2B is a G-protein coupled receptor for serotonin. The 5HT2 receptors mediate many of the central and peripheral physiologic functions of serotonin. 5HT2B plays a role in the regulation of dopamine and serotonin release, serotonin uptake and in the regulation of extracellular dopamine and serotonin levels. It also plays a role in pulmonary vasoconstriction and regulating cardiac structure and functions. 5HT2B receptors were shown to be expressed in human failing heart and have been strongly implicated in drug induced valvular heart disease.

Long Name

5-Hydroxytryptamine Receptor 2B

Entrez Gene IDs

3357 (Human); 15559 (Mouse); 29581 (Rat)

Alternate Names

5-HT(2B); 5HT2B; 5-HT2B; 5-HT-2B; 5-HT2B5-HT 2B receptor; 5-hydroxytryptamine (serotonin) receptor 2B; 5-hydroxytryptamine 2B receptor; 5-hydroxytryptamine receptor 2B; HTR2B; Serotonin receptor 2B