Human 5-HT2C APC-conjugated Antibody

Catalog #: FAB5764A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5764A-025

Detection of 5‑HT2C in HEK293 Human Cell Line Transfected with Human 5-HT2C and eGFP by Flow Cytometry.

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Human 5-HT2C APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human 5-HT2C in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 496214

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human 5-HT2C
Accession # P28335

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of 5‑HT2C in HEK293 Human Cell Line Transfected with Human 5-HT2C and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human 5-HT2C and eGFP was stained with either (A) Mouse Anti-Human 5-HT2C APC-conjugated Monoclonal Antibody (Catalog # FAB5764A) or (B) Mouse IgG_2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: 5-HT2C

5-HT2C is a 45 kDa 7TM receptor that belongs to the G-protein coupled receptor #1 family. It binds serotonin and is neuronally expressed in the limbic system of the brain. The release of dopamine due to 5-HT2C ligation regulates mood, appetite, and motivation. 5-HT2C is an important pharmacologic target in the treatment of psychosis, schizophrenia, depression, anxiety, and migrane headaches. Multiple isoforms of 5-HT2C can be generated by RNA editing. Human 5-HT2C shares 89% aa sequence identity with mouse and rat 5-HT2C.

Long Name

5-Hydroxytryptamine Receptor 2C

Entrez Gene IDs

3358 (Human); 15560 (Mouse); 25187 (Rat)

Alternate Names

5-HT1C; 5-HT-1C; 5HT2C; 5-HT2C; 5-HT-2C; 5-HT2C5-hydroxytryptamine receptor 1C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C; 5-hydroxytryptamine receptor 2C; HTR1C; HTR2C; serotonin 5-HT-2C receptor; Serotonin Receptor 2C