Human 5-HT4 Antibody

Catalog #: MAB6545 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6545
MAB6545-SP

Detection 5-HT4 in HEK293 Human Cell Line Transfected with Human 5-HT4 and eGFP by Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 5-HT4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human 5-HT4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 631769

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human 5-HT4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection 5-HT4 in HEK293 Human Cell Line Transfected with Human 5-HT4 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP was stained with (A) Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) or (B) Mouse IgG2B isotype control antibody (IC0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunohistochemistry

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5-HT4 in Human Brain. 5-HT4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT4

5-HT4 (5-hydroxytryptamine receptor 4), also known as HTR4 or 5HT4R, is an approximately 45 kDa multipass serotonin receptor that is expressed in the brain, gastrointestinal tract, bladder, vascular endothelial cells, atrium, kidney, and adrenal gland. It is involved in the regulation of learning, memory, anxiety, smooth muscle contraction, and adrenal corticosteroid secretion. Alternate splicing of human 5-HT4 generates multiple isoforms with either a 14 aa insertion in the second extracellular loop or a deletion or various substitutions in the C-terminal cytoplasmic region. Different isoforms show tissue specific expression patterns or increased activity in the absence of ligand. Human 5-HT4 shares 94% and 88% aa sequence identity with mouse and rat 5-HT4, respectively.

Long Name

5-Hydroxytryptamine Receptor 4

Entrez Gene IDs

3360 (Human)

Alternate Names

5HT4; 5-HT4; 5-HT-4; 5-HT4R; 5-hydroxytryptamine (serotonin) receptor 4,5-hydroxytryptamine receptor 4,5-HT45-hydroxytryptamine4 receptor; cardiac 5-HT4 receptor; HTR4; Serotonin receptor 4