Human 5'-Nucleotidase/CD73 APC-conjugated Antibody Summary
Trp27-Ile511
Accession # P21589
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 APC-conjugated Monoclonal Antibody (Catalog # FAB5795A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
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Detection of 5'‑Nucleotidase/CD73 in PBMC lymphocytes by Flow Cytometry. PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human 5'‑Nucleotidase/CD73 APC‑conjugated Monoclonal Antibody (Catalog # FAB5795A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: 5'-Nucleotidase/CD73
5'-Nucleotidase (also [ecto]-5'-nucleotidase/5'-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5'-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3+ Treg lymphocytes, FDCs and B cells. 5'-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5'-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5'‑Nucleotidase shares 88% aa identity with both mouse and rat 5'-Nucleotidase.
Product Datasheets
Citations for Human 5'-Nucleotidase/CD73 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation
Authors: J Liu, X Wei, J Hu, X Tan, X Kang, L Gao, N Li, X Shi, M Yuan, W Hu, M Liu
BMC Oral Health, 2021-09-24;21(1):470.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The neovascularization effect of dedifferentiated fat cells
Authors: H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto
Sci Rep, 2020-06-08;10(1):9211.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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