Human A20/TNFAIP3 Antibody

Catalog #: MAB7598 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7598
MAB7598-SP

Detection of Human A20/TNFAIP3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human A20/TNFAIP3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human A20 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human A20/TNFAIP3 (aa 440-790) is observed.

Source

Monoclonal Mouse IgG_2B Clone # 775912

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human A20
Lys91-Leu263
Accession # P21580

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

Jurkat human acute T cell leukemia cells, HepG2 human hepatocellular carcinoma cells, and NCI‑H460 human large cell lung carcinoma cells

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Colon

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human A20/TNFAIP3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NCI-H460 human large cell lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB7598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for A20/TNFAIP3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Detection of A20/TNFAIP3 in Human Colon. A20/TNFAIP3 was detected in immersion fixed paraffin-embedded sections of Human Colon using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB7598) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells in mucosal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human A20/TNFAIP3 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cells, HepG2 human hepatocellular carcinoma cells, and NCI‑H460 human large cell lung carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for A20/TNFAIP3 at approximately 101 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB7598). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A20/TNFAIP3

A20 (TNF alpha -induced protein 3) is a cytoplasmic zinc finger protein that inhibits NF kappa B activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NF kappa B-dependent gene expression by interfering with RIP- or TRAF2-mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NF kappa B signaling.

Entrez Gene IDs

7128 (Human); 21929 (Mouse)

Alternate Names

A20; A20TNFA1P2; EC 3.4.19.12; EC 6.3.2.-; MGC104522; MGC138687; OTU domain-containing protein 7C; OTUD7C; OTUD7CMGC138688; Putative DNA-binding protein A20; TNF alpha-induced protein 3; TNFA1P2; TNFAIP3; tumor necrosis factor alpha-induced protein 3; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha-induced protein 3; Zinc finger protein A20