Human A33 PE-conjugated Antibody

Catalog #: FAB3080P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3080P

Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry.

2 Images

All A33 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human A33 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse A33 is observed.

Source

Monoclonal Rat IgG_2A Clone # 402104

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human A33 PE-conjugated Monoclonal Antibody (Catalog # FAB3080P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of A33 in Colo-205 Human Cell Line by Flow Cytometry. Colo-205 human colon adenocarcinoma cell line was stained with Rat Anti-Human A33 PE-conjugated Monoclonal Antibody (Catalog # FAB3080P, filled histogram) or isotype control antibody (IC006P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: A33

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Long Name

Cell Surface A33 Antigen

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Alternate Names

A33; cell surface A33 antigen; glycoprotein A33 (transmembrane); Glycoprotein A33; GPA33; MGC129986; MGC129987; transmembrane glycoprotein A33

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human A33 PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human A33 PE-conjugated Antibody and earn rewards!

Have you used Human A33 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review