Human/Mouse/Rat/Hamster ACE-2 Antibody Summary
Gln18-Ser740
Accession # Q9BYF1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human, Mouse, and Rat ACE‑2 by Western Blot. Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
ACE‑2 in Human Kidney. ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ACE‑2 in Human Kidney. ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ACE‑2 in Hamster Lung. ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
ACE‑2 in Rat Lung. ACE‑2 was detected in immersion fixed frozen sections of rat lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in eputhelial cells in bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
ACE‑2 in Rat Lung. ACE‑2 was detected in immersion fixed paraffin-embedded sections of rat lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
ACE‑2 in Rat Kidney. ACE‑2 was detected in immersion fixed paraffin-embedded sections of rat kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human ACE‑2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE-2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ACE-2
ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).
ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)
- Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
- Crackower, M.A. et al. (2002) Nature 417:822.
- Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
- Li, W. et al. (2003) Nature 426:450.
- Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.
Product Datasheets
Citations for Human/Mouse/Rat/Hamster ACE-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Antibody-independent protection against heterologous SARS-CoV-2 challenge conferred by prior infection or vaccination
Authors: Fumagalli, V;Ravà, M;Marotta, D;Di Lucia, P;Bono, EB;Giustini, L;De Leo, F;Casalgrandi, M;Monteleone, E;Mouro, V;Malpighi, C;Perucchini, C;Grillo, M;De Palma, S;Donnici, L;Marchese, S;Conti, M;Muramatsu, H;Perlman, S;Pardi, N;Kuka, M;De Francesco, R;Bianchi, ME;Guidotti, LG;Iannacone, M;
Nature immunology
Species: Murine polyomavirus strain A3, Human hepegivirus
Sample Types: Whole Cells
Applications: Flow Cytometry -
A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection
Authors: Coria, LM;Rodriguez, JM;Demaria, A;Bruno, LA;Medrano, MR;Castro, CP;Castro, EF;Del Priore, SA;Hernando Insua, AC;Kaufmann, IG;Saposnik, LM;Stone, WB;Prado, L;Notaro, US;Amweg, AN;Diaz, PU;Avaro, M;Ortega, H;Ceballos, A;Krum, V;Zurvarra, FM;Sidabra, JE;Drehe, I;Baqué, JA;Li Causi, M;De Nichilo, AV;Payes, CJ;Southard, T;Vega, JC;Auguste, AJ;Álvarez, DE;Flo, JM;Pasquevich, KA;Cassataro, J;
Nature communications
Species: Human hepegivirus
Sample Types: Recombinant Protein
Applications: ELISA Capture -
Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection
Authors: Hou, J;Wei, Y;Zou, J;Jaffery, R;Sun, L;Liang, S;Zheng, N;Guerrero, AM;Egan, NA;Bohat, R;Chen, S;Zheng, C;Mao, X;Yi, SS;Chen, K;McGrail, DJ;Sahni, N;Shi, PY;Chen, Y;Xie, X;Peng, W;
Nature communications
Species: Human hepegivirus
Sample Types:
Applications: Western Blot -
Characterisation of the Antibody Response in Sinopharm (BBIBP-CorV) Recipients and COVID-19 Convalescent Sera from the Republic of Moldova
Authors: M Ulinici, A Sulji?, M Poggianell, R Milan Bono, K Resman Rus, A Paraschiv, AM Bonetti, M Todiras, A Corlateanu, S Groppa, E Ceban, M Petrovec, A Marcello
Vaccines, 2023-03-13;11(3):.
Species: Human
Sample Types: Whole Cells
Applications: FLOW -
Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection
Authors: AT Tang, DW Buchholz, KM Szigety, B Imbiakha, S Gao, M Frankfurte, M Wang, J Yang, P Hewins, P Mericko-Is, NA Leu, S Sterling, IA Monreal, J Sahler, A August, X Zhu, KA Jurado, M Xu, EE Morrisey, SE Millar, HC Aguilar, ML Kahn
PloS Biology, 2023-02-06;21(2):e3001989.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Mapping of functional SARS-CoV-2 receptors in human lungs establishes differences in variant binding and SLC1A5 as a viral entry modulator of hACE2
Authors: A Miluzio, A Cuomo, C Cordiglier, L Donnici, E Pesce, M Bombaci, M Conti, A Fasciani, L Terraccian, L Manganaro, M Toccafondi, A Scagliola, S Oliveto, S Ricciardi, R Grifantini, R De Frances, S Abrignani, N Manfrini, S Biffo
EBioMedicine, 2022-12-28;87(0):104390.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: Co-Immunoprecipitation, Flow Cytometry, IHC, Western Blot -
Generation and Characterization of a SARS-CoV-2-Susceptible Mouse Model Using Adeno-Associated Virus (AAV6.2FF)-Mediated Respiratory Delivery of the Human ACE2 Gene
Authors: N Tailor, BM Warner, BD Griffin, K Tierney, E Moffat, K Frost, R Vendramell, A Leung, M Willman, SP Thomas, Y Pei, SA Booth, C Embury-Hya, SK Wootton, D Kobasa
Viruses, 2022-12-28;15(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2
Authors: X Guo, J Cao, JP Cai, J Wu, J Huang, P Asthana, SKK Wong, ZW Ye, S Gurung, Y Zhang, S Wang, Z Wang, X Ge, HY Kwan, A Lyu, KM Chan, N Wong, J Huang, Z Zhou, ZX Bian, S Yuan, HLX Wong
Nature Communications, 2022-12-23;13(1):7907.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
TRPC3-Nox2 Protein Complex Formation Increases the Risk of SARS-CoV-2 Spike Protein-Induced Cardiomyocyte Dysfunction through ACE2 Upregulation
Authors: Y Kato, K Nishiyama, J Man Lee, Y Ibuki, Y Imai, T Noda, N Kamiya, T Kusakabe, Y Kanda, M Nishida
International Journal of Molecular Sciences, 2022-12-21;24(1):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling
Authors: SB Biering, FT Gomes de S, LV Tjang, F Pahmeier, C Zhu, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, DM Fox, V Ortega, AM Näär, RS Baric, SA Stanley, HC Aguilar, JD Esko, CY Chiu, JE Pak, PR Beatty, E Harris
Nature Communications, 2022-12-09;13(1):7630.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Severe acute respiratory disease in American mink experimentally infected with SARS-CoV-2
Authors: DR Adney, J Lovaglio, JE Schulz, CK Yinda, VA Avanzato, E Haddock, JR Port, MG Holbrook, PW Hanley, G Saturday, D Scott, C Shaia, AM Nelson, JR Spengler, C Tansey, CM Cossaboom, NM Wendling, C Martens, J Easley, SW Yap, SN Seifert, VJ Munster
JCI Insight, 2022-11-22;7(22):.
Species: Mink
Sample Types: Whole Tissue
Applications: IHC -
ACE2-Independent Bat Sarbecovirus Entry and Replication in Human and Bat Cells
Authors: H Guo, A Li, TY Dong, J Su, YL Yao, Y Zhu, ZL Shi, M Letko
MBio, 2022-11-21;0(0):e0256622.
Species: Bat, Human
Sample Types: Whole Cells
Applications: Neutralization -
LRRC15 inhibits SARS-CoV-2 cellular entry in trans
Authors: J Song, RD Chow, M Pena-Herna, L Zhang, SA Loeb, EY So, OD Liang, P Ren, S Chen, CB Wilen, S Lee
PloS Biology, 2022-10-13;20(10):e3001805.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Integral Membrane Protein ZMPSTE24 Protects Cells from SARS-CoV-2 Spike-Mediated Pseudovirus Infection and Syncytia Formation
Authors: K Shilagardi, ED Spear, R Abraham, DE Griffin, S Michaelis
MBio, 2022-10-05;0(0):e0254322.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner
Authors: TD Saccon, F Mousovich-, RG Ludwig, VC Carregari, AB Dos Anjos, ASC Dos Passos, MC Martini, PP Barbosa, GF de Souza, SP Muraro, J Forato, MR Amorim, RE Marques, FP Veras, E Barreto, TT Gonçalves, IM Paiva, NPB Fazolini, CMK Onodera, RB Martins Ju, PHC de Araújo, SS Batah, RMM Viana, DM de Melo, AT Fabro, E Arruda, F Queiroz Cu, TM Cunha, MAM Pretti, BJ Smith, H Marques-So, TL Knittel, GP Ruiz, GS Profeta, TCM Fontes-Cal, M Boroni, MAR Vinolo, AS Farias, PMM Moraes-Vie, JMA Bizzacchi, T Teesalu, FDM Chaim, E Cazzo, EA Chaim, JL Proença-Mó, D Martins-de, MK Osako, LO Leiria, MA Mori
Nature Communications, 2022-09-29;13(1):5722.
Species: African Green Monkey
Sample Types: Whole Cells
Applications: ICC -
Memory CD8+ T cell diversity and B cell responses correlate with protection against SARS-CoV-2 following mRNA vaccination
Authors: N Brasu, I Elia, V Russo, G Montacchie, SA Stabile, C De Intinis, F Fesi, K Gizzi, M Macagno, M Montone, B Mussolin, A Grifoni, S Faravelli, S Marchese, F Forneris, R De Frances, A Sette, V Barnaba, A Sottile, A Sapino, L Pace
Nature Immunology, 2022-09-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Metalloprotease-Dependent S2'-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2
Authors: JV Harte, SL Wakerlin, AJ Lindsay, JV McCarthy, C Coleman-Va
Viruses, 2022-09-21;14(10):.
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SARS-CoV-2 can infect human embryos
Authors: M Montano, AR Victor, DK Griffin, T Duong, N Bolduc, A Farmer, V Garg, AK Hadjantona, A Coates, FL Barnes, CG Zouves, WC Greene, M Viotti
Scientific Reports, 2022-09-14;12(1):15451.
Species: Human
Sample Types: Whole Cells
Applications: Control, ICC -
Surface translocation of ACE2 and TMPRSS2 upon TLR4/7/8 activation is required for SARS-CoV-2 infection in circulating monocytes
Authors: Y Yao, K Subedi, T Liu, N Khalasawi, CD Pretto-Ker, JW Wotring, J Wang, C Yin, A Jiang, C Fu, P Dimitrion, J Li, J Veenstra, Q Yi, K McKinnon, JE McKinnon, JZ Sexton, L Zhou, QS Mi
Cell Discovery, 2022-09-09;8(1):89.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infection
Authors: J Hou, Y Wei, J Zou, R Jaffery, S Liang, C Zheng, K Chen, PY Shi, Y Chen, X Xie, W Peng
Research square, 2022-08-15;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Development of a novel peptide to prevent entry of SARS-CoV-2 into lung and olfactory bulb cells of hACE2 expressing mice
Authors: P Su, D Zhai, AHC Wong, F Liu
Molecular Brain, 2022-08-09;15(1):71.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: Co-Immunoprecipitation, IHC, Western Blot -
SARS-CoV-2 spike N-terminal domain modulates TMPRSS2-dependent viral entry and fusogenicity
Authors: B Meng, R Datir, J Choi, CITIID-NIH, JR Bradley, KGC Smith, JH Lee, RK Gupta
Cell Reports, 2022-08-03;40(7):111220.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells
Authors: Y Zhu, KY Chew, M Wu, AC Karawita, G McCallum, LE Steele, A Yamamoto, LI Labzin, T Yarlagadda, AA Khromykh, X Wang, JDJ Sng, CJ Stocks, Y Xia, TR Kollmann, D Martino, M Joensuu, FA Meunier, G Balistreri, H Bielefeldt, AC Bowen, A Kicic, PD Sly, KM Spann, KR Short
PloS Biology, 2022-08-01;20(8):e3001728.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
Authors: H Stölting, L Baillon, R Frise, K Bonner, RJ Hewitt, PL Molyneaux, ML Gore, Breathing, WS Barclay, S Saglani, CM Lloyd
Mucosal Immunology, 2022-07-15;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A Newly Engineered A549 Cell Line Expressing ACE2 and TMPRSS2 Is Highly Permissive to SARS-CoV-2, Including the Delta and Omicron Variants
Authors: CW Chang, KM Parsi, M Somasundar, E Vanderleed, P Liu, J Cruz, A Cousineau, RW Finberg, EA Kurt-Jones
Viruses, 2022-06-23;14(7):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IgM anti-ACE2 autoantibodies in severe COVID-19 activate complement and perturb vascular endothelial function
Authors: L Casciola-R, DR Thiemann, F Andrade, MI Trejo-Zamb, EK Leonard, JB Spangler, NE Skinner, J Bailey, S Yegnasubra, R Wang, AM Vaghasia, A Gupta, AL Cox, SC Ray, RM Linville, Z Guo, PC Searson, CE Machamer, S Desiderio, LM Sauer, O Laeyendeck, BT Garibaldi, L Gao, M Damarla, PM Hassoun, JE Hooper, CA Mecoli, L Christophe, L Gutierrez-, Q Yang, D Hines, WA Clarke, RE Rothman, A Pekosz, KZ Fenstermac, Z Wang, SL Zeger, A Rosen
JCI Insight, 2022-05-09;7(9):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
FcgammaR-mediated SARS-CoV-2 infection of monocytes activates inflammation
Authors: C Junqueira, Â Crespo, S Ranjbar, LB de Lacerda, M Lewandrows, J Ingber, B Parry, S Ravid, S Clark, MR Schrimpf, F Ho, C Beakes, J Margolin, N Russell, K Kays, J Boucau, U Das Adhika, SM Vora, V Leger, L Gehrke, L Henderson, E Janssen, D Kwon, C Sander, J Abraham, MB Goldberg, H Wu, G Mehta, S Bell, AE Goldfeld, MR Filbin, J Lieberman
Nature, 2022-04-06;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization -
Two Different Therapeutic Approaches for SARS-CoV-2 in hiPSCs-Derived Lung Organoids
Authors: P Spitalieri, F Centofanti, M Murdocca, MG Scioli, A Latini, S Di Cesare, G Citro, A Rossi, A Orlandi, S Miersch, SS Sidhu, PP Pandolfi, A Botta, F Sangiuolo, G Novelli
Cells, 2022-04-05;11(7):.
Species: Human
Sample Types: Organoid
Applications: IHC -
ACE2 Receptor and Its Isoform Short-ACE2 Are Expressed on Human Spermatozoa
Authors: M Ramal-Sanc, C Castellini, C Cimini, A Taraschi, L Valbonetti, A Barbonetti, N Bernabò, B Barboni
International Journal of Molecular Sciences, 2022-03-28;23(7):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
SARS-CoV-2 infects and replicates in photoreceptor and retinal ganglion cells of human retinal organoids
Authors: Y Menuchin-L, A Schreiber, A Lecanda, A Mecate-Zam, L Brunotte, OE Psathaki, S Ludwig, T Rauen, HR Schöler
Stem Cell Reports, 2022-03-24;0(0):.
Species: Human
Sample Types: Organoid
Applications: Neutralization -
Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung
Authors: X Wang, J Luo, Z Wen, L Shuai, C Wang, G Zhong, X He, H Cao, R Liu, J Ge, R Hua, Z Sun, X Wang, J Wang, Z Bu
PloS Pathogens, 2022-02-17;18(2):e1010343.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2
Authors: S Krasemann, U Haferkamp, S Pfefferle, MS Woo, F Heinrich, M Schweizer, A Appelt-Men, A Cubukova, J Barenberg, J Leu, K Hartmann, E Thies, JL Littau, D Sepulveda-, L Zhang, K Ton, Y Liang, J Matschke, F Ricklefs, T Sauvigny, J Sperhake, A Fitzek, A Gerhartl, A Brachner, N Geiger, EM König, J Bodem, S Franzenbur, A Franke, S Moese, FJ Müller, G Geisslinge, C Claussen, A Kannt, A Zaliani, P Gribbon, B Ondruschka, W Neuhaus, MA Friese, M Glatzel, O Pless
Stem Cell Reports, 2022-01-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2
Authors: L El-Shennaw, AD Hoffmann, NK Dashzeveg, KM McAndrews, PJ Mehl, D Cornish, Z Yu, VL Tokars, V Nicolaescu, A Tomatsidou, C Mao, CJ Felicelli, CF Tsai, C Ostiguin, Y Jia, L Li, K Furlong, J Wysocki, X Luo, CF Ruivo, D Batlle, TJ Hope, Y Shen, YK Chae, H Zhang, VS LeBleu, T Shi, S Swaminatha, Y Luo, D Missiakas, GC Randall, AR Demonbreun, MG Ison, R Kalluri, D Fang, H Liu
Nature Communications, 2022-01-20;13(1):405.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Evaluation of SARS-CoV-2 entry, inflammation and new therapeutics in human lung tissue cells
Authors: J Grau-Expós, D Perea, M Suppi, N Massana, A Vergara, MJ Soler, B Trinite, J Blanco, J García-Pér, J Alcamí, A Serrano-Mo, J Rosado, V Falcó, M Genescà, MJ Buzon
PloS Pathogens, 2022-01-13;18(1):e1010171.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populations
Authors: L Helms, S Marchiano, IB Stanaway, TY Hsiang, BA Juliar, S Saini, YT Zhao, A Khanna, R Menon, F Alakwaa, C Mikacenic, ED Morrell, MM Wurfel, M Kretzler, JL Harder, CE Murry, J Himmelfarb, H Ruohola-Ba, PK Bhatraju, M Gale, BS Freedman
JCI Insight, 2021-12-22;0(0):.
Species: Human
Sample Types: Organoids
Applications: IHC -
SARS-CoV-2 uses metabotropic glutamate receptor subtype 2 as an internalization factor to infect cells
Authors: J Wang, G Yang, X Wang, Z Wen, L Shuai, J Luo, C Wang, Z Sun, R Liu, J Ge, X He, R Hua, X Wang, X Yang, W Chen, G Zhong, Z Bu
Cell Discovery, 2021-12-14;7(1):119.
Species: Human, Primate - C. aethiops
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization -
SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling
Authors: SB Biering, FTG de Sousa, LV Tjang, F Pahmeier, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, V Ortega, HC Aguilar, JD Esko, CY Chui, JE Pak, PR Beatty, E Harris
bioRxiv : the preprint server for biology, 2021-12-13;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Receptor-Binding Domain Proteins of SARS-CoV-2 Variants Elicited Robust Antibody Responses Cross-Reacting with Wild-Type and Mutant Viruses in Mice
Authors: J Shi, X Jin, Y Ding, X Liu, A Shen, Y Wu, M Peng, C Shen
Vaccines, 2021-11-24;9(12):.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Detection -
Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2
Authors: L Nguyen, KA McCord, DT Bui, KM Bouwman, EN Kitova, M Elaish, D Kumawat, GC Daskhan, I Tomris, L Han, P Chopra, TJ Yang, SD Willows, AL Mason, LK Mahal, TL Lowary, LJ West, SD Hsu, T Hobman, SM Tompkins, GJ Boons, RP de Vries, MS Macauley, JS Klassen
Nature Chemical Biology, 2021-11-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Brain Cross-Protection against SARS-CoV-2 Variants by a Lentiviral Vaccine in New Transgenic Mice
Authors: MW Ku, P Authié, M Bourgine, F Anna, A Noirat, F Moncoq, B Vesin, F Nevo, J Lopez, P Souque, C Blanc, I Fert, S Chardenoux, L Lafosse, D Cussigh, D Hardy, K Nemirov, F Guinet, F Langa Vive, L Majlessi, P Charneau
Embo Molecular Medicine, 2021-10-25;0(0):e14459.
Species: Transgenic Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
An airway organoid-based screen identifies a role for the HIF1alpha-glycolysis axis in SARS-CoV-2 infection
Authors: X Duan, X Tang, MS Nair, T Zhang, Y Qiu, W Zhang, P Wang, Y Huang, J Xiang, H Wang, RE Schwartz, DD Ho, T Evans, S Chen
Cell Reports, 2021-10-15;37(6):109920.
Species: Human
Sample Types: Organoids
Applications: IHC -
SARS-CoV-2 and its variants of concern infect human conjunctival epithelial cells and induce differential antiviral innate immune response
Authors: S Singh, G Garcia, R Shah, AA Kramerov, RE Wright, TM Spektor, AV Ljubimov, V Arumugaswa, A Kumar
The ocular surface, 2021-09-25;0(0):.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2
Authors: R Tian, AJ Samelson, VV Rezelj, M Chen, GN Ramadoss, X Guo, AM Kain, QD Tran, SA Lim, I Lui, J Nunez, SJ Rockwood, N Liu, J Carlson-St, J Oki, T Maures, K Holden, JS Weissman, JA Wells, B Conklin, M Vignuzzi, M Kampmann
bioRxiv : the preprint server for biology, 2021-09-20;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
"Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry
Authors: SP Shukla, KB Cho, V Rustagi, X Gao, X Fu, SX Zhang, B Guo, DG Udugamasoo
International Journal of Molecular Sciences, 2021-08-20;22(16):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomes
Authors: EJ Sherman, BT Emmer
Scientific Reports, 2021-08-05;11(1):15900.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Western Blot, Flow Cytometry -
Signatures in SARS-CoV-2 spike protein conferring escape to neutralizing antibodies
Authors: M Alenquer, F Ferreira, D Lousa, M Valério, M Medina-Lop, ML Bergman, J Gonçalves, J Demengeot, RB Leite, J Lilue, Z Ning, C Penha-Gonç, H Soares, CM Soares, MJ Amorim
PloS Pathogens, 2021-08-05;17(8):e1009772.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus
Authors: Y Kondo, JL Larabee, L Gao, H Shi, B Shao, CM Hoover, JM McDaniel, YC Ho, R Silasi-Man, SA Archer-Har, P Azadi, RS Srinivasan, AR Rezaie, A Borczuk, JC Laurence, F Lupu, J Ahamed, RP McEver, JF Papin, Z Yu, L Xia
JCI Insight, 2021-07-22;6(14):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Cigarette Smoke Stimulates SARS-CoV-2 Internalization by Activating AhR and Increasing ACE2 Expression in Human Gingival Epithelial Cells
Authors: CLC Almeida-da, H Matshik Da, K Liu, DM Ojcius
International Journal of Molecular Sciences, 2021-07-18;22(14):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
A human three-dimensional neural-perivascular 'assembloid' promotes astrocytic development and enables modeling of SARS-CoV-2 neuropathology
Authors: L Wang, D Sievert, AE Clark, S Lee, H Federman, BD Gastfriend, EV Shusta, SP Palecek, AF Carlin, JG Gleeson
Nature Medicine, 2021-07-09;0(0):.
Species: Human
Sample Types: Organoid
Applications: IHC -
Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2
Authors: M Zhao, PY Su, DA Castro, TN Tripler, Y Hu, M Cook, AI Ko, SF Farhadian, B Israelow, CS Dela Cruz, Y Xiong, RE Sutton, Yale IMPAC
PloS Pathogens, 2021-06-24;17(6):e1009683.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Deletion of ER-retention motif on SARS-CoV-2 spike protein reduces cell hybrid during cell-cell fusion
Authors: X Wang, CH Chen, S Badeti, JH Cho, A Naghizadeh, Z Wang, D Liu
Cell & bioscience, 2021-06-23;11(1):114.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell
Authors: M Puray-Chav, KM LaPak, TP Schrank, JL Elliott, DP Bhatt, MJ Agajanian, R Jasuja, DQ Lawson, K Davis, PW Rothlauf, Z Liu, H Jo, N Lee, K Tenneti, JE Eschbach, C Shema Mugi, EM Cousins, EW Cloer, HR Vuong, LA VanBlargan, AL Bailey, P Gilchuk, JE Crowe, MS Diamond, DN Hayes, SPJ Whelan, A Horani, SL Brody, D Goldfarb, MB Major, SB Kutluay
Cell Reports, 2021-06-23;0(0):109364.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Androgen regulation of pulmonary AR, TMPRSS2 and ACE2 with implications for sex-discordant COVID-19 outcomes
Authors: M Baratchian, JM McManus, MP Berk, F Nakamura, S Mukhopadhy, W Xu, S Erzurum, J Drazba, J Peterson, EA Klein, B Gaston, N Sharifi
Scientific Reports, 2021-05-27;11(1):11130.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Direct derivation of human alveolospheres for SARS-CoV-2 infection modeling and drug screening
Authors: T Ebisudani, S Sugimoto, K Haga, A Mitsuishi, R Takai-Toda, M Fujii, K Toshimitsu, J Hamamoto, K Sugihara, T Hishida, H Asamura, K Fukunaga, H Yasuda, K Katayama, T Sato
Cell Reports, 2021-05-19;0(0):109218.
Species: Human
Sample Types: Whole Tissue
Applications: ICC -
SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium
Authors: AZ Eriksen, R Møller, B Makovoz, SA Uhl, BR tenOever, TA Blenkinsop
Cell Stem Cell, 2021-05-17;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Deletion of ER-retention Motif on SARS-CoV-2 Spike Protein Reduces Cell Hybrid During Cell-cell Fusion
Authors: CH Chen, S Badeti, JH Cho, A Naghizadeh, X Wang, D Liu
Research square, 2021-04-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Drug repurposing screens reveal cell-type-specific entry pathways and FDA-approved drugs active against SARS-Cov-2
Authors: M Dittmar, JS Lee, K Whig, E Segrist, M Li, B Kamalia, L Castellana, K Ayyanathan, FL Cardenas-D, EE Morrisey, R Truitt, W Yang, K Jurado, K Samby, H Ramage, DC Schultz, S Cherry
Cell Reports, 2021-03-23;35(1):108959.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Characterisation of B.1.1.7 and Pangolin coronavirus spike provides insights on the evolutionary trajectory of SARS-CoV-2
Authors: SJ Dicken, MJ Murray, LG Thorne, AK Reuschl, C Forrest, M Ganeshalin, L Muir, MD Kalemera, M Palor, LE McCoy, C Jolly, GJ Towers, MB Reeves, J Grove
bioRxiv : the preprint server for biology, 2021-03-22;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
SARS-CoV-2 hijacks folate and one-carbon metabolism for viral replication
Authors: Y Zhang, R Guo, SH Kim, H Shah, S Zhang, JH Liang, Y Fang, M Gentili, CNO Leary, SJ Elledge, DT Hung, VK Mootha, BE Gewurz
Nature Communications, 2021-03-15;12(1):1676.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2
Authors: J Baggen, L Persoons, E Vanstreels, S Jansen, D Van Loover, B Boeckx, V Geudens, J De Man, D Jochmans, J Wauters, E Wauters, BM Vanaudenae, D Lambrechts, J Neyts, K Dallmeier, HJ Thibaut, M Jacquemyn, P Maes, D Daelemans
Nature Genetics, 2021-03-08;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
MRC5 cells engineered to express ACE2 serve as a model system for the discovery of antivirals targeting SARS-CoV-2
Authors: K Uemura, M Sasaki, T Sanaki, S Toba, Y Takahashi, Y Orba, WW Hall, K Maenaka, H Sawa, A Sato
Scientific Reports, 2021-03-08;11(1):5376.
Species: Human, Primate
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Pulmonary, cardiac and renal distribution of ACE2, furin, TMPRSS2 and ADAM17 in rats with heart failure: Potential implication for COVID-19 disease
Authors: EE Khoury, Y Knaney, A Fokra, S Kinaneh, Z Azzam, SN Heyman, Z Abassi
Journal of Cellular and Molecular Medicine, 2021-03-04;0(0):.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC -
An organoid-based organ-repurposing approach to treat short bowel syndrome
Authors: S Sugimoto, E Kobayashi, M Fujii, Y Ohta, K Arai, M Matano, K Ishikawa, K Miyamoto, K Toshimitsu, S Takahashi, K Nanki, Y Hakamata, T Kanai, T Sato
Nature, 2021-02-24;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Human Erythroid Progenitors Are Directly Infected by SARS-CoV-2: Implications for Emerging Erythropoiesis in Severe COVID-19 Patients
Authors: H Huerga Enc, W Grey, M Garcia-Alb, H Wood, R Ulferts, IV Aramburu, AG Kulasekara, G Mufti, V Papayannop, R Beale, D Bonnet
Stem Cell Reports, 2021-02-05;16(3):428-436.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells
Authors: X Yin, L Riva, Y Pu, L Martin-San, J Kanamune, Y Yamamoto, K Sakai, S Gotoh, L Miorin, PD De Jesus, CC Yang, KM Herbert, S Yoh, JF Hultquist, A García-Sas, SK Chanda
Cell Reports, 2021-01-12;34(2):108628.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection
Authors: C Blume, CL Jackson, CM Spalluto, J Legebeke, L Nazlamova, F Conforti, JM Perotin, M Frank, J Butler, M Crispin, J Coles, J Thompson, RA Ridley, LSN Dean, M Loxham, S Reikine, A Azim, K Tariq, DA Johnston, PJ Skipp, R Djukanovic, D Baralle, CJ McCormick, DE Davies, JS Lucas, G Wheway, V Mennella
Nature Genetics, 2021-01-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Host and viral determinants for efficient SARS-CoV-2 infection of the human lung
Authors: H Chu, B Hu, X Huang, Y Chai, D Zhou, Y Wang, H Shuai, D Yang, Y Hou, X Zhang, TT Yuen, JP Cai, AJ Zhang, J Zhou, S Yuan, KK To, IH Chan, KY Sit, DC Foo, IY Wong, AT Ng, TT Cheung, SY Law, WK Au, MA Brindley, Z Chen, KH Kok, JF Chan, KY Yuen
Nature Communications, 2021-01-08;12(1):134.
Species: Mesocricetus auratus (Hamster)
Sample Types: Whole Cells
Applications: Flow Cytometry -
SARS-CoV-2 induces human plasmacytoid pre-dendritic cell diversification via UNC93B and IRAK4
Authors: F Onodi, L Bonnet-Mad, L Meertens, L Karpf, J Poirot, SY Zhang, C Picard, A Puel, E Jouanguy, Q Zhang, J Le Goff, JM Molina, C Delaugerre, JL Casanova, A Amara, V Soumelis
bioRxiv : the preprint server for biology, 2021-01-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site
Authors: AZ Mykytyn, TI Breugem, S Riesebosch, D Schipper, PB van den Do, RJ Rottier, MM Lamers, BL Haagmans
Elife, 2021-01-04;10(0):.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men
Authors: RM Samuel, H Majd, MN Richter, Z Ghazizadeh, SM Zekavat, A Navickas, JT Ramirez, H Asgharian, CR Simoneau, LR Bonser, KD Koh, M Garcia-Kni, M Tassetto, S Sunshine, S Farahvashi, A Kalantari, W Liu, R Andino, H Zhao, P Natarajan, DJ Erle, M Ott, H Goodarzi, F Fattahi
Cell Stem Cell, 2020-11-17;27(6):876-889.e12.
Species: Human
Sample Types: Organoid
Applications: ICC -
Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD
Authors: JK Osan, SN Talukdar, F Feldmann, B Ann DeMont, K Jerome, KL Bailey, H Feldmann, M Mehedi
bioRxiv, 2020-11-12;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Effective screening of SARS-CoV-2 neutralizing antibodies in patient serum using lentivirus particles pseudotyped with SARS-CoV-2 spike glycoprotein
Authors: R Tandon, D Mitra, P Sharma, MG McCandless, SJ Stray, JT Bates, GD Marshall
Sci Rep, 2020-11-05;10(1):19076.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs
Authors: IT Lee, T Nakayama, CT Wu, Y Goltsev, S Jiang, PA Gall, CK Liao, LC Shih, CM Schürch, DR McIlwain, P Chu, NA Borchard, D Zarabanda, SS Dholakia, A Yang, D Kim, H Chen, T Kanie, CD Lin, MH Tsai, KM Phillips, R Kim, JB Overdevest, MA Tyler, CH Yan, CF Lin, YT Lin, DT Bau, GJ Tsay, ZM Patel, YA Tsou, A Tzankov, MS Matter, CJ Tai, TH Yeh, PH Hwang, GP Nolan, JV Nayak, PK Jackson
Nat Commun, 2020-10-28;11(1):5453.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
SARS-CoV-2 Mpro inhibitors and activity-based probes for patient-sample imaging
Authors: W Rut, K Groborz, L Zhang, X Sun, M Zmudzinski, B Pawlik, X Wang, D Jochmans, J Neyts, W M?ynarski, R Hilgenfeld, M Drag
Nat Chem Biol, 2020-10-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19
Authors: X Xie, AE Muruato, X Zhang, KG Lokugamage, CR Fontes-Gar, J Zou, J Liu, P Ren, M Balakrishn, T Cihlar, CK Tseng, S Makino, VD Menachery, JP Bilello, PY Shi
Nat Commun, 2020-10-15;11(1):5214.
Species: Primate
Sample Types: Whole Cells
Applications: ICC, Neutralization -
IgM autoantibodies recognizing ACE2 are associated with severe COVID-19
Authors: L Casciola-R, DR Thiemann, F Andrade, MI Trejo Zamb, JE Hooper, E Leonard, J Spangler, AL Cox, C Machamer, L Sauer, O Laeyendeck, BT Garibaldi, SC Ray, C Mecoli, L Christophe, L Gutierrez-, Q Yang, D Hines, W Clarke, RE Rothman, A Pekosz, K Fenstermac, Z Wang, SL Zeger, A Rosen
medRxiv, 2020-10-15;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Sex, androgens and regulation of pulmonary AR, TMPRSS2 and ACE2
Authors: M Baratchian, JM McManus, M Berk, F Nakamura, S Mukhopadhy, W Xu, S Erzurum, J Drazba, J Peterson, EA Klein, B Gaston, N Sharifi
bioRxiv, 2020-10-14;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids
Authors: L Pellegrini, A Albecka, DL Mallery, MJ Kellner, D Paul, AP Carter, LC James, MA Lancaster
Cell Stem Cell, 2020-10-13;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Type 2 and interferon inflammation regulate SARS-CoV-2 entry factor expression in the airway epithelium
Authors: SP Sajuthi, P DeFord, Y Li, ND Jackson, MT Montgomery, JL Everman, CL Rios, E Pruesse, JD Nolin, EG Plender, ME Wechsler, ACY Mak, C Eng, S Salazar, V Medina, EM Wohlford, S Huntsman, DA Nickerson, S Germer, MC Zody, G Abecasis, HM Kang, KM Rice, R Kumar, S Oh, J Rodriguez-, EG Burchard, MA Seibold
Nat Commun, 2020-10-12;11(1):5139.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Functional genomic screens identify human host factors for SARS-CoV-2 and common cold coronaviruses
Authors: R Wang, CR Simoneau, J Kulsuptrak, M Bouhaddou, K Travisano, JM Hayashi, J Carlson-St, J Oki, K Holden, NJ Krogan, M Ott, AS Puschnik
bioRxiv : the preprint server for biology, 2020-09-24;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response
Authors: J Huang, AJ Hume, KM Abo, RB Werder, C Villacorta, KD Alysandrat, ML Beermann, C Simone-Roa, J Lindstrom-, J Olejnik, EL Suder, E Bullitt, A Hinds, A Sharma, M Bosmann, R Wang, F Hawkins, EJ Burks, M Saeed, AA Wilson, E Mühlberger, DN Kotton
Cell Stem Cell, 2020-09-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Modeling COVID-19 with Human Pluripotent Stem Cell-Derived Cells Reveals Synergistic Effects of Anti-inflammatory Macrophages with ACE2 Inhibition Against SARS-CoV-2
Authors: F Duan, L Guo, L Yang, Y Han, A Thakur, BE Nilsson-Pa, P Wang, Z Zhang, CY Ma, X Zhou, T Han, T Zhang, X Wang, D Xu, X Duan, J Xiang, HF Tse, C Liao, W Luo, FP Huang, YW Chen, T Evans, RE Schwartz, B tenOever, DD Ho, S Chen, Q Lian, HJ Chen
Res Sq, 2020-09-15;0(0):.
Species: Human, Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Neutralization -
Antiviral Activity of Type I, II, and III Interferons Counterbalances ACE2 Inducibility and Restricts SARS-CoV-2
Authors: I Busnadiego, S Fernbach, MO Pohl, U Karakus, M Huber, A Trkola, S Stertz, BG Hale
MBio, 2020-09-10;11(5):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Neuroinvasion of SARS-CoV-2 in human and mouse brain
Authors: E Song, C Zhang, B Israelow, A Lu-Culliga, AV Prado, S Skriabine, P Lu, OE Weizman, F Liu, Y Dai, K Szigeti-Bu, Y Yasumoto, G Wang, C Castaldi, J Heltke, E Ng, J Wheeler, MM Alfajaro, E Levavasseu, B Fontes, NG Ravindra, D Van Dijk, S Mane, M Gunel, A Ring, SA Jaffar Kaz, K Zhang, CB Wilen, TL Horvath, I Plu, S Haik, JL Thomas, A Louvi, SF Farhadian, A Huttner, D Seilhean, N Renier, K Bilguvar, A Iwasaki
bioRxiv : the preprint server for biology, 2020-09-08;0(0):.
Species: Human
Sample Types: Organoid
Applications: Neutralization -
Implications for SARS-CoV-2 Vaccine Design: Fusion of Spike Glycoprotein Transmembrane Domain to Receptor-Binding Domain Induces Trimerization
Authors: T Azad, R Singaravel, MJF Crupi, T Jamieson, J Dave, EEF Brown, R Rezaei, Z Taha, S Boulton, NT Martin, A Surendran, J Poutou, M Ghahremani, K Nouri, JT Whelan, J Duong, S Tucker, JS Diallo, JC Bell, CS Ilkow
Membranes (Basel), 2020-08-30;10(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
SARS-CoV-2 Infection of Pluripotent Stem Cell-derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response
Authors: J Huang, AJ Hume, KM Abo, RB Werder, C Villacorta, KD Alysandrat, ML Beermann, C Simone-Roa, J Olejnik, EL Suder, E Bullitt, A Hinds, A Sharma, M Bosmann, R Wang, F Hawkins, EJ Burks, M Saeed, AA Wilson, E Mühlberger, DN Kotton
bioRxiv, 2020-08-06;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Cross-sectional evaluation of humoral responses against SARS-CoV-2 Spike
Authors: J Prévost, R Gasser, G Beaudoin-B, J Richard, R Duerr, A Laumaea, SP Anand, G Goyette, S Ding, H Medjahed, A Lewin, J Perreault, T Tremblay, G Gendron-Le, N Gauthier, M Carrier, D Marcoux, A Piché, M Lavoie, A Benoit, V Loungnarat, G Brochu, M Desforges, PJ Talbot, GT Gould Maul, M Côté, C Therrien, B Serhir, R Bazin, M Roger, A Finzi
bioRxiv : the preprint server for biology, 2020-07-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection
Authors: R Rathnasing, S Strohmeier, F Amanat, VL Gillespie, F Krammer, A García-Sas, L Coughlan, M Schotsaert, M Uccellini
bioRxiv, 2020-07-06;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface
Authors: J Collin, R Queen, D Zerti, B Dorgau, M Georgiou, I Djidrovski, R Hussain, JM Coxhead, A Joseph, P Rooney, S Lisgo, F Figueiredo, L Armstrong, M Lako
Ocul Surf, 2020-06-03;0(0):.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Infection of bat and human intestinal organoids by SARS-CoV-2
Authors: J Zhou, C Li, X Liu, MC Chiu, X Zhao, D Wang, Y Wei, A Lee, AJ Zhang, H Chu, JP Cai, CC Yip, IH Chan, KK Wong, OT Tsang, KH Chan, JF Chan, KK To, H Chen, KY Yuen
Nat. Med., 2020-05-13;0(0):.
Species: Bat, Human
Sample Types: Whole Cells
Applications: ICC -
Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers
Authors: IT Lee, T Nakayama, CT Wu, Y Goltsev, S Jiang, PA Gall, CK Liao, LC Shih, CM Schurch, DR McIlwain, P Chu, NA Borchard, D Zarabanda, SS Dholakia, A Yang, D Kim, T Kanie, CD Lin, MH Tsai, KM Phillips, R Kim, JB Overdevest, MA Tyler, CH Yan, CF Lin, YT Lin, DT Bau, GJ Tsay, ZM Patel, YA Tsou, CJ Tai, TH Yeh, PH Hwang, GP Nolan, JV Nayak, PK Jackson
medRxiv, 2020-05-12;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays
Authors: KHD Crawford, R Eguia, AS Dingens, AN Loes, KD Malone, CR Wolf, HY Chu, MA Tortorici, D Veesler, M Murphy, D Pettie, NP King, AB Balazs, JD Bloom
Viruses, 2020-05-06;12(5):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of Candidate COVID-19 Therapeutics using hPSC-derived Lung Organoids
Authors: Y Han, L Yang, X Duan, F Duan, BE Nilsson-Pa, TM Yaron, P Wang, X Tang, T Zhang, Z Zhao, Y Bram, D Redmond, S Houghton, D Nguyen, D Xu, X Wang, S Uhl, Y Huang, JL Johnson, J Xiang, H Wang, FC Pan, LC Cantley, BR tenOever, DD Ho, T Evans, RE Schwartz, HJ Chen, S Chen
bioRxiv, 2020-05-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Liraglutide Attenuates Non-Alcoholic Fatty Liver Disease in Mice by Regulating the Local Renin-Angiotensin System
Authors: M Yang, X Ma, X Xuan, H Deng, Q Chen, L Yuan
Front Pharmacol, 2020-04-08;11(0):432.
Species: Human, Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor.
Authors: Hoffmann M, Kleine-Weber H, Schroeder S, Kruger N, Herrler T, Erichsen S, Schiergens T, Herrler G, Wu N, Nitsche A, Muller M, Drosten C, Pohlmann S
Cell, 2020-03-05;181(0):1-10.
Species: Primate - Cercopithecus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: Neutralization -
Protective effects of the Francisella tularensis δpdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques
Authors: D Tian, A Uda, Y Ami, A Hotta, ES Park, N Nagata, N Iwata-Yosh, A Yamada, K Hirayama, K Miura, Y Koyama, M Azaki, S Morikawa
Sci Rep, 2019-06-24;9(1):9193.
Species: Rhesus Macaca (Macaque)
Sample Types: Whole Tissue
Applications: IHC-P -
Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA
Authors: E Schrom, M Huber, M Aneja, C Dohmen, D Emrich, J Geiger, G Hasenpusch, A Herrmann-J, V Kretzschma, O Mykhailyk, T Pasewald, P Oak, A Hilgendorf, D Wohlleber, HG Hoymann, D Schaudien, C Plank, C Rudolph, R Kubisch-Do
Mol Ther Nucleic Acids, 2017-04-13;7(0):350-365.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
HTCC: Broad Range Inhibitor of Coronavirus Entry
Authors: Aleksandra Milewska
PLoS ONE, 2016-06-01;11(6):e0156552.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: IHC -
Protein Kinase C-? Mediates Shedding of Angiotensin-Converting Enzyme 2 from Proximal Tubular Cells.
Authors: Fengxia Xiao, Joseph Zimpelman, Dylan Burger, Christopher Kennedy, Richard L Hébert, Kevin D Burns
Frontiers in Pharmacology, 2016-06-01;0(0):1663-9812.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Surface vimentin is critical for the cell entry of SARS-CoV.
Authors: Yu Y, Chien S, Chen I, Lai C, Tsay Y, Chang S, Chang M
J Biomed Sci, 2016-01-22;23(1):14.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Neutralization -
Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells.
Authors: Xiao F, Zimpelmann J, Agaybi S, Gurley S, Puente L, Burns K
PLoS ONE, 2014-01-15;9(1):e85958.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.
Authors: Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C
Virol J, 2013-12-23;10(0):359.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
J Virol, 2013-11-13;88(2):1293-307.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.
Authors: Ge, Xing-Yi, Li, Jia-Lu, Yang, Xing-Lou, Chmura, Aleksei, Zhu, Guangjia, Epstein, Jonathan, Mazet, Jonna K, Hu, Ben, Zhang, Wei, Peng, Cheng, Zhang, Yu-Ji, Luo, Chu-Ming, Tan, Bing, Wang, Ning, Zhu, Yan, Crameri, Gary, Zhang, Shu-Yi, Wang, Lin-Fa, Daszak, Peter, Shi, Zheng-Li
Nature, 2013-10-30;503(7477):535-8.
Species: Bat, Civet, Human
Sample Types: Whole Cells
Applications: IHC -
Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts.
Authors: Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ
PLoS ONE, 2012-04-30;7(4):e35876.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron.
Authors: Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F
J. Biol. Chem., 2010-10-21;285(53):41935-46.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2.
Authors: Glende J, Schwegmann-Wessels C, Al-Falah M, Pfefferle S, Qu X, Deng H, Drosten C, Naim HY, Herrler G
Virology, 2008-09-23;381(2):215-21.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Cell Lysates
Applications: Western Blot -
Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system.
Authors: Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S
J. Am. Coll. Cardiol., 2008-08-26;52(9):750-4.
Species: Human
Sample Types: Plasma
Applications: Neutralization -
Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.
Authors: Lu Y, Liu DX, Tam JP
Biochem. Biophys. Res. Commun., 2008-02-13;369(2):344-9.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Flow Cytometry -
Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin.
Authors: Ren W, Qu X, Li W, Han Z, Yu M, Zhou P, Zhang SY, Wang LF, Deng H, Shi Z
J. Virol., 2007-12-12;82(4):1899-907.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted.
Authors: Inoue Y, Tanaka N, Tanaka Y, Inoue S, Morita K, Zhuang M, Hattori T, Sugamura K
J. Virol., 2007-05-23;81(16):8722-9.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Lysates
Applications: Western Blot -
Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice.
Authors: Rockx B, Sheahan T, Donaldson E, Harkema J, Sims A, Heise M, Pickles R, Cameron M, Kelvin D, Baric R
J. Virol., 2007-05-16;81(14):7410-23.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Neutralization -
Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells.
Authors: de Lang A, Osterhaus AD, Haagmans BL
Virology, 2006-07-24;353(2):474-81.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: ICC -
Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells.
Authors: Tseng CT, Tseng J, Perrone L, Worthy M, Popov V, Peters CJ
J. Virol., 2005-08-01;79(15):9470-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC, Neutralization -
Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor.
Authors: Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pohlmann S
Biochem. Biophys. Res. Commun., 2004-07-09;319(4):1216-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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