Home / ADAM8 / Human ADAM8 aa 498-653 Antibody

Human ADAM8 aa 498-653 Antibody

Catalog #: MAB10311
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10311
MAB10311-SP
Detection of Human ADAM8 by Western Blot.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM8 aa 498-653 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAM8 in direct ELISAs and Western blots. Detects aa 498‑653 of rhADAM8, which corresponds to the cysteine-rich and EGF-like domains. In direct ELISAs, no cross-reactivity with recombinant human (rh) ADAM9, rhADAM10, rhADAM15, rhBACE, or rhTACE is observed.
Source
Monoclonal Mouse IgG1 Clone # 143338
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ADAM8 ectodomain
Asp158-Ser653
Accession # P78325
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAM8 aa 158-653 see our available Western blot detection antibodies. Does not detect Recombinant Human ADAM8 aa 158-497.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ADAM8 antibody by Western Blot. View Larger

Detection of Human ADAM8 by Western Blot. Western blot shows Recombinant Human ADAM8 aa158-653 and Recombinant Human ADAM8 aa 158-497. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ADAM8 Monoclonal Antibody (Catalog # MAB10311) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ADAM8 aa158-653 at approximately 65 kDa (as indicated). The antibody did not detect ADAM8 aa 158-497, indicating it is specific for aa 498-653. This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM8

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).

References
  1. Yoshiyama, K. et al. (1997) Genomics 41:56.
  2. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
  3. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  4. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
  5. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
  6. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.
Long Name
A Disintegrin and Metalloprotease-like Domain 8
Entrez Gene IDs
101 (Human)
Alternate Names
a disintegrin and metalloproteinase domain 8; ADAM 8; ADAM metallopeptidase domain 8; ADAM8; CD156a antigen; CD156a; CD156human leukocyte differentiation antigen; Cell surface antigen MS2; EC 3.4.24; EC 3.4.24.-; MGC134985; MS2; MS2disintegrin and metalloproteinase domain-containing protein 8

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Citations for Human ADAM8 aa 498-653 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Extracellular Vesicle-Based Detection of Pancreatic Cancer
    Authors: Yesim Verel-Yilmaz, Juan Pablo Fernández, Agnes Schäfer, Sheila Nevermann, Lena Cook, Norman Gercke et al.
    Frontiers in Cell and Developmental Biology
  2. miR-720 is a downstream target of an ADAM8-induced ERK signaling cascade that promotes the migratory and invasive phenotype of triple-negative breast cancer cells
    Authors: SG Das, M Romagnoli, ND Mineva, S Barillé-Ni, P Jézéquel, M Campone, GE Sonenshein
    Breast Cancer Res, 2016-04-02;18(1):40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding.
    Authors: Gomez-Gaviro M, Dominguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernandez A, Mollinedo F, Sanchez-Madrid F, Diaz-Gonzalez F
    J. Immunol., 2007-06-15;178(12):8053-63.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot
  4. Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes.
    Authors: Johansson MW, Lye MH, Barthel SR, Duffy AK, Annis DS, Mosher DF
    Am. J. Respir. Cell Mol. Biol., 2004-06-25;31(4):413-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  5. ADAM 8 expression in invasive breast cancer promotes tumor dissemination and metastasis
    Authors: Mathilde Romagnoli, Nora D Mineva, Michael Polmear, Catharina Conrad, Srimathi Srinivasan, Delphine Loussouarn et al.
    EMBO Molecular Medicine

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