Human ADAMTS1 Propeptide Antibody

Catalog #: AF3079
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3079
AF3079-SP

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Human ADAMTS1 Propeptide Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTS1 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with mature recombinant human ADAMTS1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ADAMTS1
Leu50-Arg252
Accession # Q9UHI8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ADAMTS1 (Catalog # 2197-AD)

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAMTS1 (Catalog # 2197‑AD), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ADAMTS1 in Human Placenta. ADAMTS1 was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Goat Anti-Human ADAMTS1 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3079) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS1

ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multi-domain structure (1-3). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha -2-macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of cofactor such as fibulin-1 (8). The aggrecanase activity can be inhibited using 5 mM 1,10 Phenanthroline. ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF₁₆₅ and to inhibit fibroblast migration at high concentrations by binding to FGF-2 (11, 12). The purified rhADAMTS1 starts at the N-terminus of the catalytic domain and ends in the beginning of the spacer region.

References

Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
Porter, S. et al. (2005) Biochem. J. 386:15.
Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
Kuno, K. et al. (2000) FEBS Lett. 478:241.
Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
Lee, N., et al. (2005) J. Biol. Chem. 280:34796.
Shindo, T., et al. (2000) J. Clin. Invest. 105:1345.
Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 1

Entrez Gene IDs

9510 (Human)

Alternate Names

a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; ADAM-TS 1; ADAMTS1; ADAM-TS1; ADAMTS-1; C3-C5; METH1; METH-1

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Citation for Human ADAMTS1 Propeptide Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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