Human ADAMTS10 Antibody

Catalog #: MAB5270 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5270
MAB5270-SP

All ADAMTS10 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAMTS10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTS10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAMTS1, 3, 4, 5, 12, 13, 15, 16, or L2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 496708

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS10
Phe26-Gly1102
Accession # Q9H324

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human ADAMTS10 under non-reducing conditions only

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAMTS10, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS10

ADAMTS10 belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent metalloproteases. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. Mutations in the ADAMTS10 gene may cause autosomal recessive Weill-Marchesani syndrome. The amino acid sequence of human ADAMTS10 is 100%, 96% and 94% identical to that of chimpanzee, dog and mouse.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 10

Entrez Gene IDs

81794 (Human); 224697 (Mouse); 362847 (Rat)

Alternate Names

a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 10; ADAM metallopeptidase with thrombospondin type 1 motif, 10; ADAM-TS 10; ADAMTS10; ADAMTS-10; ADAM-TS10A disintegrin and metalloproteinase with thrombospondin motifs 10; EC 3.4.24.-; EC 3.4.24.82; WMS; zinc metalloendopeptidase