Human ADAMTS3 Antibody

Catalog #: AF5465 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5465
AF5465-SP

All ADAMTS3 Antibodies

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Human ADAMTS3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 3% cross-reactivity with recombinant human (rh) ADAMTS4 and less than 1% cross-reactivity with rhADAMTS1, rhADAMTS5, rhADAMTS13, and rhADAMTSL1.2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS3
Ser19-Cys712
Accession # O15072

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ADAMTS3

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAMTS3, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS3

ADAMTS3 (A disintegrin and metalloprotease with thrombospondin motifs 3; also PC II-NP) is a 140-150 kDa member of the ADAMTS family of Zn metalloproteases. It is expressed by osteoblasts, chrondrocytes, myoepithelium and fibroblasts, and participates in collagen maturation. Together with ADAMTS2 and TS14, ADAMTS3 is known to process the N-terminus of procollagen. In particular, it cleaves the N-terminal globular region of collagen I and II, leading to fibril formation. Mature human proADAMTS3 is a secreted, 1185 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 38-201), a peptidase M12B domain (aa 256-460), a disintegrin region (aa 470-550), four TSP type I sequences (aa 551-1015), and a PLAC domain (aa 1015-1054). There are two potential splice variants. One shows a 38 aa substitution for aa 169-1205, while another shows an alternative start site at Met171. Over aa 19-712, human ADAMTS3 shares 91% aa identity with mouse ADAMTS3.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 3

Entrez Gene IDs

9508 (Human); 330119 (Mouse); 305253 (Rat)

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 3; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 3; ADAM metallopeptidase with thrombospondin type 1 motif, 3; ADAM-TS 3; ADAMTS3; ADAM-TS3; ADAMTS-3; ADAMTS-4; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.14; KIAA0366PC II-NP; PC II-NP; Procollagen II amino propeptide-processing enzyme; Procollagen II N-proteinase; zinc metalloendopeptidase