Human Aggrecan Antibody

Catalog #: MAB113041 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB113041-100
MAB113041-SP
Human Aggrecan ELISA Standard Curve.
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Human Aggrecan Antibody Summary

Species Reactivity
Human
Specificity
Detects human Aggrecan G1-IGD-G2 Domains in direct ELISA.
Source
Monoclonal Mouse IgG1 Clone # 179514
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aggrecan
Val20-Gly675
Accession # NP_037359
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA
This antibody functions as an ELISA detection antibody when paired with human anti ADAMTS-5 Monoclonal Antibody (Catalog # MAB11304).This product is intended for assay development on various assay platforms requiring antibody pairs.

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Aggrecan DuoSet ELISA Kit (Catalog # DY1220) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human Aggrecan ELISA Standard Curve. Recombinant Human Aggrecan protein was serially diluted 2-fold and captured by Mouse Anti-Human Aggrecan Monoclonal Antibody (MAB11304) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Aggrecan Monoclonal Antibody (Catalog # MAB113041) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aggrecan

Aggrecan, also known as aggrecan 1, chondroitin sulfate proteoglycan, and large aggregating proteoglycan, is encoded by the AGC1 gene with gene aliases of SEDK; CSPG1; MSK16; CSPGCP (1). As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases (2). The deduced amino acid sequence of human aggrecan core protein consists of 2415 residues and predicts a signal peptide and domains of G1, IGD, G2, KS, CS-1, CS-2 and G3 (3). Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the C-terminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250 kDa core protein, aggrecan monomers exist as a 1,000 to 2,000 kDa molecule. In addition, aggrecan monomers interact with hyaluronan through their G1 domain, resulting in larger aggregates containing 10 to 100 aggrecan monomers on a hyaluronan backbone (2). Aggrecan can be cleaved by MMPs and ADAMTSs at the Asn360-Phe361 and Glu392-Ala393 bond in the IGD (residues are numbered based on Accession # NP_037359), respectively (2). Inhibition of ADATMS4 and ADAMTS5 cleavage prevents aggrecan degradation in osteoarthritic cartilage, while mice with aggrecan resistant to MMP cleavage do not accumulate aggrean and develop normally (2, 4).

References
  1. Doege. K.J. et al. (1991) J. Biol. Chem. 266:894.
  2. Malfait, A.-M. et al. (2002) J. Biol. Chem. 277:22201.
  3. Caterson, B. et al. (2000) Matrix Biol. 19:333.
  4. Little, C.B. et al. (2005) Mol. Cel. Biol. 25:3388.
Entrez Gene IDs
176 (Human)
Alternate Names
ACAN; AGC1; AGC1SEDK; aggrecan core protein; Aggrecan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1; CSPG1aggrecan 1; CSPGCP; large aggregating proteoglycan; MSK16; MSK16AGCAN; SEDK

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