Human ALCAM/CD166 Biotinylated Antibody

Catalog #: BAM6561
1 Citation Datasheet / COA / SDS

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BAM6561

All ALCAM/CD166 Antibodies

Product Details

Citations (1)

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Human ALCAM/CD166 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ALCAM/CD166 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) ALCAM, recombinant human (rh) BCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, rmMadCAM, or rmOCAM is observed.

Source

Monoclonal Mouse IgG₁ Clone # 105902

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # AAB59499

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL)
under non-reducing conditions only

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood monocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM (activated leukocyte cell adhesion molecule), designated CD166 and also called MEMD and SC‑1/DM‑GRASP/BEN in the chicken, is a 100‑110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte‑derived dendritic cells (1, 2). Human ALCAM cDNA encodes 583 amino acid (aa), including signal peptide (27 aa), extracellular domain (ECD, 500 aa) with two V‑type and three C2‑type Ig‑like domains, transmembrane (22 aa) and cytoplasmic (34 aa) domains (1). Human ALCAM ECD shares 93%, 95% and 96% aa sequence identity with mouse/rat, bovine and porcine/equine ALCAM, respectively. A 570 aa isoform lacks aa 503‑515, while a 555 aa form lacks most of the cytoplasmic domain. A secreted isoform in endothelial cells that is truncated at aa 133 (sALCAM) antagonizes full‑length ALCAM (3, 4). ALCAM mediates low‑affinity adhesion with itself or the cysteine‑rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1‑11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM‑expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (2, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on melanoma cell lines appears to be pro‑metastatic, but anti‑metastatic activity has been reported in breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM‑L1, both in the developing retina and invasive melanoma (3, 12).

References

Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
Zimmerman, A.W. et al. (2006) Blood 107:3212.
van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
King, J.A. et al. (2010) Mol. Cancer 9:266.
Buhusi, M. et al. (2009) J. Neurosci. 29:15630.

Long Name

Activated Leukocyte Cell Adhesion Molecule

Entrez Gene IDs

214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)

Alternate Names

activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

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Citation for Human ALCAM/CD166 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Lung Basal Stem Cells Rapidly Repair DNA Damage Using the Error-Prone Nonhomologous End-Joining Pathway
Authors: CE Weeden, Y Chen, SB Ma, Y Hu, G Ramm, KD Sutherland, GK Smyth, ML Asselin-La
PLoS Biol, 2017-01-26;15(1):e2000731.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry