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Human ALCAM/CD166 PE-conjugated Antibody

Catalog #: FAB6561P
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB6561P
Detection of ALCAM/CD166 in Human Whole Blood Lymphocytes by Flow Cytometry.
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Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ALCAM/CD166 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ALCAM in Western blots. Shows approximately 50% cross-reactivity with recombinant mouse OCAM and no cross-reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.
Source
Monoclonal Mouse IgG1 Clone # 105902
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # Q13740
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ALCAM/CD166 antibody in Human Whole Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of ALCAM/CD166 in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human ALCAM/CD166 PE-conjugated Monoclonal Antibody (Catalog # FAB6561P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

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Citations for Human ALCAM/CD166 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. B4GALNT1 induces angiogenesis, anchorage independence growth and motility, and promotes tumorigenesis in melanoma by induction of ganglioside GM2/GD2
    Authors: H Yoshida, L Koodie, K Jacobsen, K Hanzawa, Y Miyamoto, M Yamamoto
    Sci Rep, 2020-01-27;10(1):1199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
    Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
    Stem Cell Res Ther, 2019-11-04;10(1):316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Methionine is a metabolic dependency of tumor-initiating cells
    Authors: Z Wang, LY Yip, JHJ Lee, Z Wu, HY Chew, PKW Chong, CC Teo, HY Ang, KLE Peh, J Yuan, S Ma, LSK Choo, N Basri, X Jiang, Q Yu, AM Hillmer, WT Lim, TKH Lim, A Takano, EH Tan, DSW Tan, YS Ho, B Lim, WL Tam
    Nat. Med., 2019-05-06;25(5):825-837.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Reduced CD146 expression promotes tumorigenesis and cancer stemness in colorectal cancer through activating Wnt/beta-catenin signaling.
    Authors: Liu D, DU L, Chen D, Ye Z, Duan H, Tu T, Feng J, Yang Y, Chen Q, Yan X
    Oncotarget, 2016-06-28;7(26):40704-40718.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CD166/ALCAM expression is characteristic of tumorigenicity and invasive and migratory activities of pancreatic cancer cells.
    Authors: Fujiwara K, Ohuchida K, Sada M, Horioka K, Ulrich C, Shindo K, Ohtsuka T, Takahata S, Mizumoto K, Oda Y, Tanaka M
    PLoS ONE, 2014-09-15;9(9):e107247.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Establishment of highly tumorigenic human colorectal cancer cell line (CR4) with properties of putative cancer stem cells.
    Authors: Rowehl, Rebecca, Burke, Stephani, Bialkowska, Agnieszk, Pettet, Donald W, Rowehl, Leahana, Li, Ellen, Antoniou, Eric, Zhang, Yuanhao, Bergamaschi, Roberto, Shroyer, Kenneth, Ojima, Iwao, Botchkina, Galina I
    PLoS ONE, 2014-06-12;9(6):e99091.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Activated leukocyte cell adhesion molecule (CD166): an "inert" cancer stem cell marker for non-small cell lung cancer?
    Authors: Tachezy M, Zander H, Wolters-Eisfeld G, Muller J, Wicklein D, Gebauer F, Izbicki J, Bockhorn M
    Stem Cells, 2014-06-01;32(6):1429-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
    Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
    PLoS ONE, 2014-01-29;9(1):e84417.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012-10-09;7(10):e47187.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. CD166(pos) subpopulation from differentiated human ES and iPS cells support repair of acute lung injury.
    Authors: Soh, Boon Sen, Zheng, Dahai, Li Yeo, Julie Su, Yang, Henry He, Ng, Shi Yan, Wong, Lan Hion, Zhang, Wencai, Li, Pin, Nichane, Massimo, Asmat, Atasha, Wong, Poo Sing, Wong, Peng Che, Su, Lin Lin, Mantalaris, Sakis A, Lu, Jia, Xian, Wa, McKeon, Frank, Chen, Jianzhu, Lim, Elaine H, Lim, Bing
    Mol Ther, 2012-09-11;20(12):2335-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection, Flow Cytometry
  11. Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines.
    Authors: Kondo S, Wakisaka N, Muramatsu M
    J. Virol., 2011-08-17;85(21):11255-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011-05-19;6(5):e19988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.
    Authors: Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX
    Am. J. Physiol., Cell Physiol., 2010-02-24;298(5):C1217-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC

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