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Human Alkaline Phosphatase/ALPL Antibody

Catalog #: MAB1448
9 Citations 2 Reviews Datasheet / COA / SDS
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MAB1448
MAB1448-SP
Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.
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Product Details
Citations (9)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Alkaline Phosphatase/ALPL Antibody Summary

Species Reactivity
Human
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Source
Monoclonal Mouse IgG1 Clone # B4-78
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
BG01V human embryonic stem cells and Whole blood granulocytes.
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Alkaline Phosphatase/ALPL antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Immunocytochemistry Alkaline Phosphatase and Oct‑3/4 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline Phosphatase/ALPL and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Alkaline Phosphatase/ALPL antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Alkaline Phosphatase/ALPL in Human Kidney. Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow Cytometry View Larger

Detection of Alkaline Phosphatase/ALPL in Whole blood granulocytes by Flow Cytometry. Whole blood granulocytes were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References
  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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Citations for Human Alkaline Phosphatase/ALPL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D In Vitro Models
    Authors: S Martin-Igl, L Milian, M Sancho-Tel, R Salvador-C, JJ Martín de, C Carda, M Mata
    Stem Cells International, 2022-03-04;2022(0):4910399.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells
    Authors: J Camps, H Grosemans, R Gijsbers, C Maes, M Sampaolesi
    Int J Mol Sci, 2019-03-05;20(5):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
    Authors: Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A
    PLoS ONE, 2015-03-12;10(3):e0118499.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Osteodifferentiated mesenchymal stem cells from bone marrow and adipose tissue express HLA-G and display immunomodulatory properties in HLA-mismatched settings: implications in bone repair therapy.
    Authors: Montespan F, Deschaseaux F, Sensebe L, Carosella E, Rouas-Freiss N
    J Immunol Res, 2014-04-30;2014(0):230346.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Flow Cytometry
  5. Cementum- and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig's epithelial root sheath cells.
    Authors: Bai Y, Bai Y, Matsuzaka K, Hashimoto S, Fukuyama T, Wu L, Miwa T, Liu X, Wang X, Inoue T
    Bone, 2011-03-01;48(6):1417-26.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro.
    Authors: Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS
    Bone, 2009-04-18;45(2):309-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. In vivo impact of a 4 bp deletion mutation in the DLX3 gene on bone development.
    Authors: Choi SJ, Roodman GD, Feng JQ, Song IS, Amin K, Hart PS, Wright JT, Haruyama N, Hart TC
    Dev. Biol., 2008-10-25;325(1):129-37.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold.
    Authors: Valarmathi MT, Yost MJ, Goodwin RL, Potts JD
    Biomaterials, 2008-03-04;29(14):2203-16.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  9. Bone marrow-derived osteoblast progenitor cells in circulating blood contribute to ectopic bone formation in mice.
    Authors: Otsuru S, Tamai K, Yamazaki T, Yoshikawa H, Kaneda Y
    Biochem. Biophys. Res. Commun., 2007-01-10;354(2):453-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Human Alkaline Phosphatase/ALPL Antibody
By Anonymous on 09/09/2021
Application: IHC Sample Tested: Ovarian cancer tissue Species: Human
Immunohistochemistry Human Alkaline Phosphatase/ALPL Antibody MAB1448
Human Alkaline Phosphatase/ALPL Antibody
By Anonymous on 02/16/2020
Application: IHC Sample Tested: Liver cancer tissue Species: Human
Immunohistochemistry Human Alkaline Phosphatase/ALPL Antibody MAB1448