Human Alkaline Phosphatase/ALPP Antibody

Catalog #: MAB5905 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5905
MAB5905-SP

Detection of Human Alkaline Phosphatase/ALPP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Alkaline Phosphatase/ALPP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Alkaline Phosphatase/ALPP in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Intestinal Alkaline Phosphatase/ALPI or rhAlkaline Phsophatase/ALPL is observed.

Source

Monoclonal Mouse IgG_2B Clone # 696108

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Alkaline Phosphatase/ALPP
Ile23-Asp506
Accession # P05187

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Alkaline Phosphatase/ALPP, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Alkaline Phosphatase/ALPP by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # MAB5905) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Alkaline Phosphatase/ALPP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPP

Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPP gene encodes the 67 kDa placental isozyme, also known as PLAP. In comparison, ALPI, ALPL, and ALPPL2 encode intestinal, liver/bone/kidney, and placental-like or germ cell APs, respectively. The placental alkaline phosphatase is a glycosyl phosphatidylinositol (GPI) anchored protein that is expressed on the cell surface as a homodimer. Human APs are ectopically expressed in several cancers, and their serum levels are useful tumor markers. Mature human Alkaline Phosphatase/ALPP shares 74% amino acid sequence homology with the mouse and rat enzymes.

Long Name

Alkaline Phosphatase, Placental Type

Entrez Gene IDs

250 (Human); 11650 (Mouse); 367308 (Rat)

Alternate Names

Alkaline phosphatase Regan isozyme; Alkaline Phosphatase; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; alkaline phosphatase, placental; alkaline phosphomonoesterase; ALP; ALPP; EC 3.1.3.1; FLJ61142; glycerophosphatase; PALP; Placental alkaline phosphatase 1; PLAP; PLAP-1; Regan isozyme; SEAP