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Human alpha 2-Macroglobulin Antibody

Catalog #: MAB1938
2 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1938
MAB1938-SP
Detection of Human alpha 2‑Macroglobulin by Western Blot.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha 2-Macroglobulin Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha 2-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with rhC5a or rmC5a.
Source
Monoclonal Mouse IgG1 Clone # 257316
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human plasma-derived alpha 2-Macroglobulin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human a<sub>2</sub>-Macroglobulin antibody by Western Blot. View Larger

Detection of Human alpha 2‑Macroglobulin by Western Blot. Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human a<sub>2</sub>-Macroglobulin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human alpha 2‑Macroglobulin by Simple WesternTM. Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 2-Macroglobulin

Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.

Entrez Gene IDs
2 (Human)
Alternate Names
A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7

Product Datasheets

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Citations for Human alpha 2-Macroglobulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Fecal host biomarkers predicting severity of Clostridioides difficile infection
    Authors: M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh
    JCI Insight, 2021-01-11;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  2. Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors.
    Authors: Telford G, Brown AP, Kind A, English JS, Pritchard DI
    Br. J. Dermatol., 2010-11-29;164(1):192-6.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

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Reviews for Human alpha 2-Macroglobulin Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human alpha 2-Macroglobulin Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human alpha 2-Macroglobulin Antibody MAB1938
Human alpha 2-Macroglobulin Antibody
By Anonymous on 01/09/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human alpha 2-Macroglobulin Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human and Mouse