Human alpha 2-Macroglobulin Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human alpha 2‑Macroglobulin by Western Blot. Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human alpha 2‑Macroglobulin by Simple WesternTM. Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for a2-Macroglobulin at approximately 178 kDa (as indicated) using 1 µg/mL of Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2-Macroglobulin
Human alpha 2-macroglobulin (h alpha 2M) is a serum glycoprotein that has sequence similarity to other members of the alpha 2M family including complement components C3, C4 and C5 (1). alpha 2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) (2). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha 2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha 2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha 2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.
- Sottrup-Jensen, L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:9.
- Kan, C.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:2282.
Product Datasheets
Citations for Human alpha 2-Macroglobulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Post-Translational Regulation And Proteolytic Activity Of The Metalloproteinase Adamts8
Authors: S Santamaria, DR Martin, X Dong, K Yamamoto, SS Apte, J Ahnström
The Journal of Biological Chemistry, 2021-10-21;0(0):101323.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ratio of Alpha 2-Macroglobulin Levels in Cerebrospinal Fluid and Serum: An Expression of Neuroinflammation in Acute Disseminated Encephalomyelitis
Authors: Y Suzuki, K Hashimoto, K Hoshi, H Ito, Y Kariya, K Miyazaki, M Sato, Y Kawasaki, M Yoshida, T Honda, Y Hashimoto, M Hosoya
Pediatr. Neurol., 2019-05-11;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis.
Authors: Dalli, Jesmond, Norling, Lucy V, Montero-Melendez, Trinidad, Federici Canova, Donata, Lashin, Hazem, Pavlov, Anton M, Sukhorukov, Gleb B, Hinds, Charles, Perretti, Mauro
EMBO Mol Med, 2013-12-15;6(1):27-42.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter.
Authors: Kim JC, Kim JY, Yeom SR, Jeong BY, Hwang HZ, Park KJ, Lee SW
Proteomics, 2008-09-01;8(17):3632-44.
Species: Rat
Sample Types: Serum
Applications: Western Blot
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