Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

Catalog #: AF7159 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7159
AF7159-SP

Detection of Human alpha -N-terminal Methyltransferase 1A/ METTL11A by Western Blot.

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All alpha-N-terminal Methyltransferase 1A/METTL11A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha -N-terminal Methyltransferase 1A/METTL11A in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human alpha -N-terminal Methyltransferase 1A/METTL11A
Thr2-Arg223
Accession # Q9BV86

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human alpha -N-terminal Methyltransferase 1A/ METTL11A by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human a-N-terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-N-terminal Methyltransferase 1A/ METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-N-terminal Methyltransferase 1A/METTL11A

METTL11A, also named N‑terminal RCC1 methyltransferase (NRMT), is an alpha‑N‑methyltransferase that methylates the N‑terminal amino group of target proteins containing the N‑terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N‑terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman‑like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide‑exchange factor) is the first protein for which any biological function of alpha‑N‑methylation by NRMT has been identified (3, 4). The multi‑spindle phenotype associated with either NRMT knockdown or methylation‑defective RCC1 mutants demonstrated the importance of alpha‑N‑methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers.

References

Webb, K. J. et al. (2010) Biochemistry 49:5225.
Christine, S. E. et al. (2010) Nature 466:1125.
Chen, T. et al. (2007) Nature Cell Biol. 9:596.
Hao, Y. et al. (2008) J. Cell. Biol. 182:827.

Long Name

alpha-N-terminal Protein Methyltransferase 1A

Entrez Gene IDs

28989 (Human); 66617 (Mouse); 362103 (Rat)

Alternate Names

AD-003; alpha N-terminal protein methyltransferase 1A; C9orf32; chromosome 9 open reading frame 32; EC 2.1.1.-; methyltransferase like 11A; Methyltransferase-like protein 11A; METTL11A; NRMT; N-terminal RCC1 methyltransferase; NTM1A; NTMT1; X-Pro-Lys N-terminal protein methyltransferase 1A