Human alpha-Smooth Muscle Actin Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1420R-100UG
Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry
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Product Details
Citations (5)
FAQs
Reviews (1)

Human alpha-Smooth Muscle Actin Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human  alpha -Smooth Muscle Actin.
Source
Monoclonal Mouse IgG2A Clone # 1A4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha -smooth muscle actin (1)
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of alpha -Smooth Muscle Actin antibody in Human Blood Monocytes antibody by Flow Cytometry View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry Human peripheral blood monocytes were stained with Mouse Anti-Human alpha -Smooth Muscle Actin Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC1420R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References
  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human alpha-Smooth Muscle Actin Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Spatial multi-omics of human skin reveals KRAS and inflammatory responses to spaceflight
    Authors: Park, J;Overbey, EG;Narayanan, SA;Kim, J;Tierney, BT;Damle, N;Najjar, D;Ryon, KA;Proszynski, J;Kleinman, A;Hirschberg, JW;MacKay, M;Afshin, EE;Granstein, R;Gurvitch, J;Hudson, BM;Rininger, A;Mullane, S;Church, SE;Meydan, C;Church, G;Beheshti, A;Mateus, J;Mason, CE;
    Nature communications
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Sci-Seq of Human Fetal Salivary Tissue Introduces Human Transcriptional Paradigms and a Novel Cell Population
    Authors: Devon Duron Ehnes, Ammar Alghadeer, Sesha Hanson-Drury, Yan Ting Zhao, Gwen Tilmes, Julie Mathieu et al.
    Frontiers in Dental Medicine
  3. Single-nucleus and spatial transcriptome profiling of pancreatic cancer identifies multicellular dynamics associated with neoadjuvant treatment
    Authors: Hwang WL, Jagadeesh KA, Guo JA Et al.
    Nat Genet
  4. 3D Printed Bioreactor Enabling the Pulsatile Culture of Native and Angioplastied Large Arteries
    Authors: Rolando S. Matos, Davide Maselli, John H. McVey, Christian Heiss, Paola Campagnolo
    Frontiers in Cardiovascular Medicine
  5. System-wide transcriptome damage and tissue identity loss in COVID-19 patients
    Authors: Park J, Foox J, Hether T Et al.
    Cell Rep Med

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Human alpha-Smooth Muscle Actin Alexa Fluor® 647-conjugated Antibody
By Anonymous on 05/20/2023
Application: Flow Sample Tested: iPS2 human induced pluripotent stem cells,NIH-3T3 mouse embryonic fibroblast cell line,Umbilical vein endothelial cells Species: Human

I have tried alpha-SMA actin antibody on different cell lines with different conditions, but apparently it is not specific to SMA.