Human Aminopeptidase P1/XPNPEP1 Antibody

Catalog #: MAB2970 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2970
MAB2970-SP

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Human Aminopeptidase P1/XPNPEP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Aminopeptidase P1/XPNPEP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) XPNPEP1, rmXPNPEP2, or recombinant human XPNPEP2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 409111

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Aminopeptidase P1/XPNPEP1
Met1-Gln622
Accession # Q9NQW7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Aminopeptidase P1/XPNPEP1 (Catalog # 2970-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase P1/XPNPEP1

The human XPNPEP1 gene encodes aminopeptidase P1 (APP1), which is also known as X-prolyl aminopeptidase with gene aliases of SAMP, XPNPEP, XPNPEPL, and XPNPEPL1 (1‑3). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, aminopeptidase P2, aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (4). It is a soluble enzyme, in contrast to the GPI-anchored APP2 encoded by XPNPEP2 (5). Human APP1 is widely expressed (3). APP1 removes a N-terminal amino acid from a peptide that contains a Pro residue at the second position. The amino acid sequence of human APP1 is 99%, 97%, 95%, 74%, and 73% identical to that of canine, bovine, mouse/rat, Xenopus, and zebrafish, respectively.

References

Cottrell, G.S. et al. (2000) Biochemistry 39:15121.
Sprinkle, T.J. et al. (2000) Arch. Biochem. Biophys. 378:51.
Vanhoof, G. et al. (1997) Cytogenet. Cell Genet. 78:275.
Turner, A.J. and G.S. Cottrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 931, Elsevier Academic Press, San Diego.
Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934, Elsevier Academic Press, San Diego.

Entrez Gene IDs

7511 (Human)

Alternate Names

Aminoacylproline aminopeptidase; Aminopeptidase P1; APP1; Cytosolic aminopeptidase P; cytosolic; EC 3.4.11.9; SAMP; xaa-Pro aminopeptidase 1; XPNPEP1; XPNPEPLX-prolyl aminopeptidase (aminopeptidase P)-like; X-prolyl aminopeptidase (aminopeptidase P) 1, soluble; X-prolyl aminopeptidase 1, soluble