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Human Aminopeptidase P1/XPNPEP1 Antibody

Catalog #: AF2970
1 Citation Datasheet / COA / SDS
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AF2970
AF2970-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Aminopeptidase P1/XPNPEP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Aminopeptidase P1/XPNPEP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) XPNPEP2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Aminopeptidase P1/XPNPEP1 Met1-His623 Accession # NP_065116
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Aminopeptidase P1/XPNPEP1 (Catalog # 2970-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase P1/XPNPEP1 (Catalog # 2970-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Aminopeptidase P1/XPNPEP1

The human XPNPEP1 gene encodes aminopeptidase P1 (APP1), which is also known as X-prolyl aminopeptidase with gene aliases of SAMP, XPNPEP, XPNPEPL, and XPNPEPL1 (1-3). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, aminopeptidase P2, aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (4). It is a soluble enzyme, in contrast to the GPI-anchored APP2 encoded by XPNPEP2 (5). Human APP1 is widely expressed (3). The purified rhAPP1 is an active aminopeptidase, removing a N-terminal amino acid from a peptide that contains a Pro residue at the second position. The amino acid sequence of human APP1 is 99%, 97%, 95%, 74% and 73% identical to that of canine, bovine, mouse/rat, Xenopus and zebrafish, respectively.

References
  1. Cottrell, G.S. et al. (2000) Biochemistry 39:15121.
  2. Sprinkle, T.J. et al. (2000) Arch. Biochem. Biophys. 378:51.
  3. Vanhoof, G. et al. (1997) Cytogenet. Cell Genet. 78:275.
  4. Turner, A.J. and G.S. Cottrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 931, Elsevier Academic Press, San Diego. 
  5. Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934, Elsevier Academic Press, San Diego. 
Entrez Gene IDs
7511 (Human)
Alternate Names
Aminoacylproline aminopeptidase; Aminopeptidase P1; APP1; Cytosolic aminopeptidase P; cytosolic; EC 3.4.11.9; SAMP; xaa-Pro aminopeptidase 1; XPNPEP1; XPNPEPLX-prolyl aminopeptidase (aminopeptidase P)-like; X-prolyl aminopeptidase (aminopeptidase P) 1, soluble; X-prolyl aminopeptidase 1, soluble

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Citation for Human Aminopeptidase P1/XPNPEP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Flavivirus Infection Impairs Peroxisome Biogenesis and Early Antiviral Signaling.
    Authors: You J, Hou S, Malik-Soni N, Xu Z, Kumar A, Rachubinski R, Frappier L, Hobman T
    J Virol, 2015-09-30;89(24):12349-61.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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