Human Angiopoietin-1 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Saliva, Human Milk
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 611 | 1217 | 2502 | 619 | 1223 | 2496 |
Standard Deviation | 20.8 | 24.2 | 67 | 42.4 | 71.5 | 113 |
CV% | 3.4 | 2 | 2.7 | 6.8 | 5.8 | 4.5 |
Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 587 | 1179 | 2339 | 620 | 1230 | 2533 |
Standard Deviation | 14 | 28.5 | 76.1 | 39.5 | 67.1 | 141 |
CV% | 2.4 | 2.4 | 3.3 | 6.4 | 5.5 | 5.6 |
Recovery
The recovery of Angiopoietin-1 spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 104 | 97-110 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: B7-H3
B7-H3 is an immunoglobulin superfamily protein that is expressed by osteoblasts, fibroblasts, ciliated respiratory epithelial cells, anterior pituitary progenitor cells, first trimester extravillous trophoblasts, and multiple tumor cells. It is induced on activated dendritic cells (DC), skeletal muscle myocytes, and monocytes. B7-H3 can both stimulate and inhibit immune cell activation. As a co-stimulator, B7-H3 augments macrophage activation and promotes proliferation and cytokine secretion by CD4+ and CD8+ T cells. As a co-inhibitor, tumor cell B7-H3 blocks the cytolytic activity of NK cells, limits cytokine production by activated T cells, and imparts an immunosuppressive phenotype to dendritic cells. B7-H3 can interact with TREML2/TLT-2 to enhance T cell activation including the CD8+ T cell-mediated lysis of tumor cells.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Angiopoietin-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
51
Citations: Showing 1 - 10
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Increased Angiopoietin-1 and -2 levels in human vitreous are associated with proliferative diabetic retinopathy
Authors: T Tsai, M Alwees, MA Asaad, J Theile, V Kakkassery, HB Dick, T Schultz, SC Joachim
PLoS ONE, 2023-01-20;18(1):e0280488.
Species: Human
Sample Types: Vitreous Humor
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Serum angiopoietin-1 concentration does not distinguish patients with ischaemic stroke from those presenting to hospital with ischaemic stroke mimics
Authors: JV Moxon, AK Kraeuter, J Phie, S Juliano, G Anderson, G Standley, C Sealey, RP White, J Golledge
BMC cardiovascular disorders, 2022-11-04;22(1):462.
Species: Human
Sample Types: Serum
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Upregulation of the oestrogen target gene SIX1 is associated with higher growth speed and decreased survival in HCV-positive women with hepatocellular carcinoma
Authors: RM Critelli, F Milosa, A Romanzi, S Lasagni, G Marcelli, L Di Marco, A Pivetti, F Schepis, D Romagnoli, S Mancarella, F Dituri, ML Martinez-C, G Giannelli, E Villa
Oncology Letters, 2022-09-21;24(5):395.
Species: Human
Sample Types: Serum
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Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients
Authors: M Hultström, K Fromell, A Larsson, B Persson, B Nilsson, SE Quaggin, C Betsholtz, R Frithiof, M Lipcsey, M Jeansson
Biomedicines, 2022-06-06;10(6):.
Species: Human
Sample Types: Plasma
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Platelet-derived growth factor BB is reduced in endometrial endothelial cells of women with abnormal uterine bleeding-endometrial disorder
Authors: S Biswas Shi, Q Lu, D Sun, H Hou, JN Bulmer, BA Innes, DK Hapangama, GE Lash
Oncogene, 2022-02-13;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells
Authors: L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet
Leukemia, 2021-09-23;0(0):.
Species: Human
Sample Types: Plasma
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Mucopolysaccharide polysulfate promotes microvascular stabilization and barrier integrity of dermal microvascular endothelial cells via activation of the angiopoietin-1/Tie2 pathway
Authors: S Fujiwara-S, Y Ueda, M Fujikawa, M Osaki, N Yamanaka, T Matsumoto
Journal of dermatological science, 2021-06-02;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Defined MSC exosome with high yield and purity to improve regenerative activity
Authors: JY Kim, WK Rhim, YI Yoo, DS Kim, KW Ko, Y Heo, CG Park, DK Han
Journal of tissue engineering, 2021-04-20;12(0):2041731421100.
Species: Human
Sample Types: Cell Lysates
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Interleukin-6 as an enhancer of anti-angiogenic therapy for ovarian clear cell carcinoma
Authors: T Seki, N Yanaihara, JS Shapiro, M Saito, J Tabata, R Yokomizo, D Noguchi, T Kuroda, A Kawabata, J Suzuki, K Takahashi, H Matsuzawa, M Miyake, M Takenaka, Y Iida, S Yanagida, A Okamoto
Scientific Reports, 2021-04-08;11(1):7689.
Species: Human
Sample Types: Cell Culture Supernates
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Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells
Authors: AA Hastreiter, GG Dos Santos, EN Makiyama, EWC Santos, P Borelli, RA Fock
The Journal of nutritional biochemistry, 2021-03-08;0(0):108626.
Species: Mouse
Sample Types: Cell Culture Supernates
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Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p
Authors: C Gao, Z Gong, D Wang, J Huang, Y Qian, M Nie, W Jiang, X Liu, H Luo, J Yuan, T Xiang, S An, W Quan, H Wei, J Zhang, R Jiang
Aging (Albany NY), 2019-12-16;11(24):12147-12164.
Species: Human
Sample Types: Serum
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MicroRNA-411 and Its 5'-IsomiR Have Distinct Targets and Functions and Are Differentially Regulated in the Vasculature under Ischemia
Authors: RVCT van der Kw, T Woudenberg, PHA Quax, AY Nossent
Mol. Ther., 2019-10-07;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Systemic Inflammation Precedes Microalbuminuria in Diabetes
Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
Kidney Int Rep, 2019-06-21;4(10):1373-1386.
Species: Human
Sample Types: Serum
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Microcirculatory perfusion disturbances following cardiac surgery with cardiopulmonary bypass are associated with in vitro endothelial hyperpermeability and increased angiopoietin-2 levels
Authors: NAM Dekker, ALI van Leeuwe, WWJ van Strien, J Majolée, R Szulcek, ABA Vonk, PL Hordijk, C Boer, CE van den Br
Crit Care, 2019-04-11;23(1):117.
Species: Human
Sample Types: Plasma
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Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
Authors: M Varas-Godo, S Acuña-Gall, S Venegas-Du, C Hill, A Caceres-Ve, O Realini, LJ Monteiro, G Zavala, M Khoury, R Romero, G Rice, SE Illanes
Stem Cells Int, 2019-01-27;2019(0):1916542.
Species: Human
Sample Types: Cell Culture Supernates
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Different patterns of inflammatory and angiogenic factors are associated with peritoneal small solute transport and peritoneal protein clearance in peritoneal dialysis patients
Authors: Y Shi, H Yan, J Yuan, H Zhang, J Huang, Z Ni, J Qian, W Fang
BMC Nephrol, 2018-05-23;19(1):119.
Species: Human
Sample Types: Serum
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Tie2 as a novel key factor of microangiopathy in systemic sclerosis
Authors: F Moritz, J Schniering, JHW Distler, RE Gay, S Gay, O Distler, B Maurer
Arthritis Res. Ther., 2017-05-25;19(1):105.
Species: Human
Sample Types: Serum
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Alteration Analysis of Bone Marrow Mesenchymal Stromal Cells from De Novo Acute Myeloid Leukemia Patients at Diagnosis
Authors: L Desbourdes, J Javary, T Charbonnie, N Ishac, J Bourgeais, A Iltis, JC Chomel, A Turhan, F Guilloton, K Tarte, MV Demattei, E Ducrocq, F Rouleux-Bo, E Gyan, O Hérault, J Domenech
Stem Cells Dev., 2017-04-10;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
Lab. Invest., 2017-04-10;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Differential regulation of blood flow induced neovascularisation and mural cell recruitment by VEGF and angiopoietin signalling
Authors: David O Bates
J. Physiol. (Lond.), 2017-02-02;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
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Angiopoietin-2, Angiopoietin-1 and subclinical cardiovascular disease in Chronic Kidney Disease
Sci Rep, 2016-12-19;6(0):39400.
Species: Human
Sample Types: Serum
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Circulating Angiopoietin-1 Is Not a Biomarker of Disease Severity or Prognosis in Pulmonary Hypertension
PLoS ONE, 2016-11-01;11(11):e0165982.
Species: Human
Sample Types: Plasma
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Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
Sci Rep, 2016-07-04;6(0):29029.
Species: Human
Sample Types: Cell Culture Supernates
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TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
Sci Adv, 2016-04-01;2(4):e1501290.
Species: Human
Sample Types: Cell Lysates
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Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
PLoS ONE, 2015-08-26;10(8):e0136205.
Species: Human
Sample Types: Plasma
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EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
Authors: Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S
Stem Cells, 2015-06-23;33(9):2838-49.
Species: Human
Sample Types: Cell Culture Supernates
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Angiopoietin-2 and angiopoietin-2/angiopoietin-1 ratio as indicators of potential severity of Plasmodium vivax malaria in patients with thrombocytopenia.
Authors: Gomes L, Alves-Junior E, Rodrigues-Jesus C, Nery A, Gasquez-Martin T, Fontes C
PLoS ONE, 2014-10-02;9(10):e109246.
Species: Human
Sample Types: Serum
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Serum angiopoietin-2 and soluble VEGFR-2 levels predict malignancy of ovarian neoplasm and poor prognosis in epithelial ovarian cancer.
Authors: Sallinen H, Heikura T, Koponen J, Kosma V, Heinonen S, Yla-Herttuala S, Anttila M
BMC Cancer, 2014-09-23;14(0):696.
Species: Human
Sample Types: Serum
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Impaired function of the Tie-2 receptor contributes to vascular leakage and lethality in anthrax.
Proc Natl Acad Sci U S A, 2012-06-04;109(25):10024-9.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Serum
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Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
BMC Cancer, 2011-07-05;11(0):286.
Species: Human
Sample Types: Serum
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Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
Diabetologia, 2011-06-10;54(9):2358-67.
Species: Human
Sample Types: Serum
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CD34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans.
Authors: Sobrino T, Arias S, Perez-Mato M, Agulla J, Brea D, Rodriguez-Yanez M, Castillo J
J. Neurosci. Res., 2011-04-12;89(7):979-85.
Species: Human
Sample Types: Serum
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Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
Crit. Care Med., 2011-04-01;0(0):.
Species: Human
Sample Types: Plasma
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Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
Authors: Boucherat O, Franco-Montoya ML, Delacourt C, Martinovic J, Masse V, Elie C, Thebaud B, Benachi A, Bourbon JR
Am. J. Physiol. Lung Cell Mol. Physiol., 2010-03-26;298(6):L849-56.
Species: Human
Sample Types: Tissue Homogenates
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Colorectal resection is associated with persistent proangiogenic plasma protein changes: postoperative plasma stimulates in vitro endothelial cell growth, migration, and invasion.
Authors: Shantha Kumara HM, Feingold D, Kalady M, Dujovny N, Senagore A, Hyman N, Cekic V, Whelan RL
Ann. Surg., 2009-06-01;249(6):973-7.
Species: Human
Sample Types: Plasma
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Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
Exp. Hematol., 2009-06-01;37(6):673-8.
Species: Human
Sample Types: Plasma
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Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation.
Authors: Hurliman AK, Speroff L, Stouffer RL, Patton PE, Lee A, Molskness TA
Fertil. Steril., 2009-05-23;93(5):1493-9.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Serum
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Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors.
Authors: Serefhanoglu S, Goker H, Buyukasik Y, Turgut M, Sayinalp N, Haznedaroglu IC, Aksu S, Akman U, Ozcebe OI
Ann. Hematol., 2008-12-11;88(7):667-71.
Species: Human
Sample Types: Plasma
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Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
Exp. Hematol., 2008-05-27;36(8):1035-46.
Species: Human
Sample Types: Cell Culture Supernates
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Structural stability of neoangiogenic intramyocardial microvessels supports functional recovery in chronic ischemic myocardium.
Authors: Shim WS, Lim SY, Li SQ, Gu Y, Ong HC, Song IC, Chuah SC, Wong P
J. Mol. Cell. Cardiol., 2008-04-27;45(1):70-80.
Species: Porcine
Sample Types: Tissue Homogenates
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Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
Ann. Surg., 2008-02-01;247(2):320-6.
Species: Human
Sample Types: Plasma
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Relationship of soluble CD40 ligand to vascular endothelial growth factor, angiopoietins, and tissue factor in atrial fibrillation: a link among platelet activation, angiogenesis, and thrombosis?
Authors: Choudhury A, Freestone B, Patel J, Lip GY
Chest, 2007-12-01;132(6):1913-9.
Species: Human
Sample Types: Plasma
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Angiopoietin-2 as a contributing factor of exercise-induced bronchoconstriction in asthmatic patients receiving inhaled corticosteroid therapy.
Authors: Kanazawa H, Tochino Y, Asai K
J. Allergy Clin. Immunol., 2007-11-26;121(2):390-5.
Species: Human
Sample Types: Sputum
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Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia.
Authors: Lee CY, Tien HF, Hu CY, Chou WC, Lin LI
Br. J. Cancer, 2007-09-11;97(7):877-82.
Species: Human
Sample Types: Plasma
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Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
Br. J. Haematol., 2007-06-01;137(6):560-8.
Species: Human
Sample Types: Serum
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Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
Stem Cells, 2007-04-19;25(8):1862-71.
Species: Human
Sample Types: Cell Culture Supernates
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Roles of angiopoietin-1 and angiopoietin-2 on airway microvascular permeability in asthmatic patients.
Authors: Kanazawa H, Nomura S, Asai K
Chest, 2007-04-01;131(4):1035-41.
Species: Human
Sample Types: Sputum
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Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers.
Authors: Niedzwiecki S, Stepien T, Kopec K, Kuzdak K, Komorowski J, Krupinski R, Stepien H
Cytokine, 2007-03-19;36(5):291-5.
Species: Human
Sample Types: Serum
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Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
Authors: Caine GJ, Ryan P, Lip GY, Blann AD
Cancer Lett., 2007-01-19;251(2):296-301.
Species: Human
Sample Types: Plasma
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Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
Cancer Lett., 2006-08-07;248(1):131-6.
Species: Human
Sample Types: Plasma
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