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Human Angiostatin Antibody

Catalog #: AF226
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF226
AF226-SP
Product Details
Citations (8)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiostatin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Angiostatin in direct ELISAs and Western blots. This antibody will also recognize human plasminogen.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Angiostatin Kringle 1-3
Val98-Ser358 with Cys188Ser & Cys316Ser substitutions
Accession # P00747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Angiostatin Kringle 1-3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiostatin

Angiostatin is a 38-45 kDa proteolytic fragment of plasminogen, a 92-100 kDa glycosylated blood zymogen that serves as the precursor for plasmin.  Mature human plasminogen is 713 amino acids (aa) in length (aa 98-810).  It contains five N-terminal kringle domains (aa 103-561) plus one peptidase S1 domain (aa 581-808). Upon cleavage by tPA between Arg580 and Val581, plasminogen is converted into the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots.  Plasminogen is also cleaved by MMPs which generate multiple fragments.  Angiostatin has documented antiangiogenic and antitumor activity, likely mediated by a downregulation of Bcl2, and an upregulation of thrombospondin-1.  Over aa 98-358, human angiostatin shares 81% aa identity with mouse angiostatin.  

Entrez Gene IDs
5340 (Human); 18815 (Mouse)
Alternate Names
Angiostatin; HAE4

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Citations for Human Angiostatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes
    Authors: S El Chamy M, MY Icimoto, PMS Melo, A Budu, R Coimbra, ML Gazarini, AK Carmona
    Malar. J., 2020-08-26;19(1):302.
    Species: Human, Parasite - Plasmodium falciparum
    Sample Types: Cell Fraction, Cell Lystates
    Applications: Western Blot
  2. Temporal and pharmacological characterization of angiostatin release and generation by human platelets: implications for endothelial cell migration.
    Authors: Radziwon-Balicka A, Moncada de la Rosa C, Zielnik B, Doroszko A, Jurasz P
    PLoS ONE, 2013-03-15;8(3):e59281.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunogold Electron Microscopy, Western Blot
  3. Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid.
    Authors: Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K
    Gene Ther., 2009-12-01;16(12):1405-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Oncolytic herpesvirus with secretable angiostatic proteins in the treatment of human lung cancer cells.
    Authors: Yang CT, Lin YC, Lin CL, Lu J, Bu X, Tsai YH, Jia WW
    Anticancer Res., 2005-05-01;25(3):2049-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. Adeno-associated virus 2-mediated antiangiogenic cancer gene therapy: long-term efficacy of a vector encoding angiostatin and endostatin over vectors encoding a single factor.
    Authors: Ponnazhagan S, Mahendra G, Kumar S, Shaw DR, Stockard CR, Grizzle WE, Meleth S
    Cancer Res., 2004-03-01;64(5):1781-7.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  6. Angiostatin and matrix metalloprotease expression following ischemic acute renal failure.
    Authors: Basile DP, Fredrich K, Weihrauch D, Hattan N, Chilian WM
    Am. J. Physiol. Renal Physiol., 2004-01-06;286(5):F893-902.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Generation and role of angiostatin in human platelets.
    Authors: Jurasz P, Alonso D, Castro-Blanco S, Murad F, Radomski MW
    Blood, 2003-07-10;102(9):3217-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  8. A truncated plasminogen activator inhibitor-1 protein blocks the availability of heparin-binding vascular endothelial growth factor A isoforms.
    Authors: Mulligan-Kehoe MJ, Kleinman HK, Drinane M, Wagner RJ, Wieland C, Powell RJ
    J. Biol. Chem., 2002-10-14;277(50):49077-89.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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