Home / Annexin A4 / Human Annexin A4 Antibody

Human Annexin A4 Antibody

Catalog #: MAB4146
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4146
MAB4146-SP
Detection of Human, Mouse, and Rat Annexin A4 by Western Blot.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Annexin A4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Annexin A4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Annexin A1, A2, A3, A5, A6, A8, A9, A10, A11, or 13A is observed.
Source
Monoclonal Mouse IgG2B Clone # 694624
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Annexin A4
Met1-Asp319
Accession # P09525
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Annexin A4 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Annexin A4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry Annexin A4 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Annexin A4 in Human Kidney. Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and the nuclear envelope of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Annexin A4

Annexin A4 (ANXA4), also known as Lipocortin IV, placental anticoagulant protein II, and zymogen granule membrane-associated protein of 36 kDa (ZAP36), is a member of the Annexin protein family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Human Annexin A4 shares 92% aa sequence identity with mouse and rat Annexin A4.

Entrez Gene IDs
307 (Human); 11746 (Mouse); 79124 (Rat)
Alternate Names
AIV; Annexin A4; annexin IV (placental anticoagulant protein II); Annexin IV; annexin-4; ANX4; ANX4Carbohydrate-binding protein p33/p41; ANXA4; Chromobindin-4; chromobindin-4,35-beta calcimedin; DKFZp686H02120; Endonexin I; Lipocortin IV; MGC75105; P32.5; PAP-II; PIG28; Placental anticoagulant protein II; PP4-X; proliferation-inducing gene 28; proliferation-inducing protein 28; Protein II; ZAP36

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Annexin A4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Simultaneous membrane binding of Annexin A4 and A5 suppresses 2D lattice formation while maintaining curvature induction
    Authors: A Mularski, SL Sønder, ASB Heitmann, J Nylandsted, AC Simonsen
    Journal of colloid and interface science, 2021-05-18;600(0):854-864.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair
    Authors: TL Boye, K Maeda, W Pezeshkian, SL Sønder, SC Haeger, V Gerke, AC Simonsen, J Nylandsted
    Nat Commun, 2017-11-20;8(1):1623.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Annexin A4 Antibody

There are currently no reviews for this product. Be the first to review Human Annexin A4 Antibody and earn rewards!

Have you used Human Annexin A4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review