Home / Apolipoprotein A-II/ApoA2 / Human Apolipoprotein A-II/ApoA2 Antibody

Human Apolipoprotein A-II/ApoA2 Antibody

Catalog #: MAB4215
1 Citation Datasheet / COA / SDS
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MAB4215
MAB4215-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein A-II/ApoA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ApoA2 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) ApoA1 or rhApoE.
Source
Monoclonal Rat IgG2A Clone # 395906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ApoA2
Gln24-Gln100
Accession # P02652
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Apolipoprotein A-II/ApoA2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-II/ApoA2

Apolipoprotein A2 (ApoA2) is a major protein component of serum HDL. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. ApoA2 can form disulfide-linked 17 kDa homodimers and heterodimers with other apolipoproteins. Mature human ApoA2 shares 96% aa sequence identity with chimpanzee ApoA2 and 48% - 66% aa sequence identity with bovine, equine, mouse, and rat ApoA2.

Entrez Gene IDs
336 (Human); 11807 (Mouse); 25649 (Rat)
Alternate Names
Alp-2; APOA2; apoAII; Apo-AII; ApoA-II; Apolipoprotein A2; Apolipoprotein AII; Apolipoprotein A-II; Hdl-1

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Citation for Human Apolipoprotein A-II/ApoA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Amphipathic alpha-helices in apolipoproteins are crucial to the formation of infectious hepatitis C virus particles.
    Authors: Fukuhara T, Wada M, Nakamura S, Ono C, Shiokawa M, Yamamoto S, Motomura T, Okamoto T, Okuzaki D, Yamamoto M, Saito I, Wakita T, Koike K, Matsuura Y
    PLoS Pathog, 2014-12-11;10(12):e1004534.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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