Human APP/Protease Nexin II (Amyloid beta) Antibody

Catalog #: MAB96182 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB96182-100
MAB96182-SP

Human Amyloid beta (aa1-40) ELISA Standard Curve.

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Human APP/Protease Nexin II (Amyloid beta) Antibody Summary

Species Reactivity

Human

Specificity

Detects Human APP/Protease Nexin II (amino acids 1-11 of Amyloid beta ) in direct ELISAs.

Source

Monoclonal Mouse IgG Clone # AB42-5

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant protein to Fibrillar Amyloid beta 1-42 peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Amyloid beta (aa1-40) Monoclonal Antibody (Catalog # MAB96181 or Catalog # MAB96181R) or Mouse Anti-Human Amyloid beta (aa1-42) Monoclonal Antibody (Catalog # MAB9618 or Catalog # MAB9618R).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Amyloid beta (aa1-40) Quantikine ELISA Kit (Catalog # DAB140B) or Human Amyloid beta (aa1-42) Quantikine ELISA Kit (Catalog # DAB142) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Amyloid beta (aa1-40) ELISA Standard Curve. Recombinant Human Amyloid beta protein was serially diluted 2-fold and captured by Mouse Anti-Human Amyloid beta (aa1-40) Monoclonal Antibody (Catalog # MAB96181or Catalog # MAB96181R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

ELISA

View Larger

Human Amyloid beta (aa1-42) ELISA Standard Curve. Recombinant Human Amyloid beta protein was serially diluted 2-fold and captured by Mouse Anti-Human Amyloid beta (aa1-42) Monoclonal Antibody (Catalog # MAB9618or Catalog # MAB9618R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APP

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Long Name

Amyloid Precursor Protein

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Alternate Names

amyloid beta (A4) precursor protein-binding, family B, member 2; amyloid beta A4 precursor protein-binding family B member 2; Amyloid beta precursor protein; Amyloid beta; APP; beta Amyloid; Protease Nexin II