Human ATM Antibody

Catalog #: MAB22901 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB22901
MAB22901-SP

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Human ATM Antibody Summary

Species Reactivity

Human

Specificity

Detects human ATM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human ATR is observed.

Source

Monoclonal Mouse IgG_2B Clone # 664703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ATM
Arg2138-Arg2400
Accession # Q13315

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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ATM in HeLa Human Cell Line. ATM was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human ATM Monoclonal Antibody (Catalog # MAB22901) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATM

ATM (Ataxia Telangiectasia Mutated) is a 350-370 kDa member of the ATM subfamily, PI3/PI4-kinase family of enzymes. It is ubiquitously expressed, and serves as a DNA damage sensor. ATM is activated via autophosphorylation at double strand breaks. Following activation, multiple substrates are phosphorylated, including Chk2, and ATR is recruited and activated as part of an integrated repair circuit. Human ATM is 3056 amino acids (aa) in length. It contains one FAT (focal adhesion targeting) domain (aa 1960-2566), a PI‑3/PI‑4 kinase catalytic domain (aa 2712-2962) and a second, C-terminal FAT domain (aa 3024-3056). There are at least six Ser and four Thr utilized phosphorylation sites, and one critical acetylation activation site at Lys3016. There are at least four potential splice variants. One shows a Trp substitution for aa 536-3056, a second contains an eight aa substitution for aa 2506-3056, a third possesses a five aa substitution for aa 1637-3056, while a fourth contains a premature truncation after Lys2756. Over aa 2138-2400, human ATM shares 82% aa identity with mouse ATM.

Long Name

Ataxia Telangiectasia-mutated

Entrez Gene IDs

472 (Human); 11920 (Mouse)

Alternate Names

AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutated; ATC; ATD; ATDC; ATE; ATM serine/threonine kinase; ATM; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL