Human Axin-2 Antibody

Catalog #: MAB6078
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6078
MAB6078-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Axin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Axin-2 in direct ELISAs. In this format, no cross-reactivity with recombinant human Axin-1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 354214

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Axin-2
Tyr550-Gln760
Accession # Q9Y2T1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Axin‑2 in SW480 Human Cell Line. Axin-2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human Axin-2 Monoclonal Antibody (Catalog # MAB6078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axin-2

Axin-2, also known as Conductin, is a 95 kDa cytoplasmic protein that inhibits Wnt signaling and promotes epithelial-mesenchymal transition. Axin 2 binds to beta -catenin, glycogen synthase kinase-3 (GSK-3 beta ), and the tumor suppressor APC. Loss of Axin-2 function is linked to carcinogenesis as well as abnormal bone and tooth development. Within aa 550-760, human Axin-2 shares 82% aa sequence identity with mouse and rat Axin-2.

Long Name

Axis Inhibitor 2

Entrez Gene IDs

8313 (Human)

Alternate Names

AXIL; axin 2; Axin2; Axin-2; Axin-like protein; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Axin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Organoid modeling of human fetal lung alveolar development reveals mechanisms of cell fate patterning and neonatal respiratory disease
Authors: K Lim, APA Donovan, W Tang, D Sun, P He, JP Pett, SA Teichmann, JC Marioni, KB Meyer, AH Brand, EL Rawlins
Cell Stem Cell, 2022-12-08;30(1):20-37.e9.
Species: Human
Sample Types: Organoid
Applications: IHC