Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody
Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody Summary
Ala81-Leu285
Accession # Q9Y275
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of BAFF/BLyS/TNFSF13B in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin (filled histogram) or resting (open histogram), were stained with Mouse Anti-Human BAFF/BLyS/TNFSF13B PE-conjugated Monoclonal Antibody (Catalog # IC1241P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
Background: BAFF/BLyS/TNFSF13B
BAFF, also known as TALL-1, BLyS, and THANK, is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between Arg133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF Receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6 - 8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).
- Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
- Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
- Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
- Liu, Y. et al. (2002) Cell 108:383.
- Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
- Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
- Thompson, J.S. et al. (2001) Science 293:2108.
- Ng, L.G. et al. (2004) J. Immunol. 173:807.
- Roschke, V. et al. (2002) J. Immunol. 169:4314.
- Batten, M. et al. (2000) J. Exp. Med. 192:1453.
- Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
Product Datasheets
Citations for Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma.
Authors: Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
Br. J. Dermatol., 2012-07-05;167(2):359-67.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha.
Authors: Nishio M, Endo T, Tsukada N, Ohata J, Kitada S, Reed JC, Zvaifler NJ, Kipps TJ
Blood, 2005-04-28;106(3):1012-20.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody and earn rewards!
Have you used Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image