Human Bcl-2 Minus C-Terminus Antibody Summary
Met1-Asp211
Accession # P10415
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Bcl‑2 Minus C-Terminus by Western Blot. Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.1-0.5 µg/mL of Mouse Anti-Human Bcl-2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB827) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl-2 Minus C-Terminus at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Bcl‑2 by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for Bcl‑2 at approximately 24 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Bcl‑2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB827). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-2
Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
Product Datasheets
Citations for Human Bcl-2 Minus C-Terminus Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53
Authors: MM Mabrouk Za, HA Sahyon, NAN Hanafy, MA El-Kemary
Oncogene, 2022-05-29;14(6):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Aurora kinase A inhibition reverses the Warburg effect and elicits unique metabolic vulnerabilities in glioblastoma
Authors: TTT Nguyen, E Shang, C Shu, S Kim, A Mela, N Humala, A Mahajan, HW Yang, HO Akman, CM Quinzii, G Zhang, MA Westhoff, G Karpel-Mas, JN Bruce, P Canoll, MD Siegelin
Nature Communications, 2021-09-01;12(1):5203.
Species: Human
Sample Types: Cell Lysates
Applications: Protein Capillary Electrophore -
Epigenetic Targeting of Mcl-1 Is Synthetically Lethal with Bcl-xL/Bcl-2 Inhibition in Model Systems of Glioblastoma
Authors: E Shang, TTT Nguyen, C Shu, MA Westhoff, G Karpel-Mas, MD Siegelin
Cancers (Basel), 2020-08-01;12(8):.
Species: Human
Sample Types: Cell Lysates, Protein
Applications: Western Blot -
Mediation of electronegative low-density lipoprotein signaling by LOX-1: a possible mechanism of endothelial apoptosis.
Authors: Lu J, Yang JH, Burns AR, Chen HH, Tang D, Walterscheid JP, Suzuki S, Yang CY, Sawamura T, Chen CH
Circ. Res., 2009-01-15;104(5):619-27.
Species: Bovine
Sample Types: Cell Lysates
Applications: Western Blot -
Interactions between important regulatory proteins and human alphaB crystallin.
Authors: Ghosh JG, Shenoy AK, Clark JI
Biochemistry, 2007-05-08;46(21):6308-17.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA-Based Protein Pin Array
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