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Human Bcl-9 Antibody

Catalog #: AF3996
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3996
AF3996-SP
Detection of Human Bcl‑9 by Western Blot.
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Citations (1)
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Human Bcl-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human Bcl-9 in Western blots.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Bcl‑9 antibody by Western Blot. View Larger

Detection of Human Bcl‑9 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Bcl-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3996) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Bcl-9 at approximately 150 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Bcl-9

Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514‑517 and 970‑973), and one poly-Ala segment (aa 900‑903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391‑1426. Over aa 1009‑1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.

Long Name
B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling
Entrez Gene IDs
607 (Human); 77578 (Mouse); 310704 (Rat)
Alternate Names
B-cell CLL/lymphoma 9 protein; B-cell CLL/lymphoma 9; B-cell lymphoma 9 protein; Bcl9; Bcl-9; BCL9L; LGS; MGC131591; Protein legless homolog

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Citation for Human Bcl-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Wnt status-dependent oncogenic role of BCL9 and BCL9L in hepatocellular carcinoma
    Authors: Nicole Huge, Maria Sandbothe, Anna K. Schröder, Amelie Stalke, Marlies Eilers, Vera Schäffer et al.
    Hepatology International

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