Human Bcl9-2 Antibody Summary
Met38-Val206
Accession # Q86UU0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Bcl9‑2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Bcl9-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4967) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Bcl9-2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl9-2
Bcl9-2 (B-cell lymphoma 9-like protein; also Bcl9L and B9L) is a 200-220 kDa transcriptional regulator that belongs to the Bcl9 family of proteins. It is expressed in multiple tissues, particularly breast, and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl9-2 and Bcl9 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl9-2 is 1499 amino acids (aa) in length. It contains two Pro-rich regions (aa 280-493 and 891-1378). There are four potential alternate start sites at Met879, Met1000, Met708 and Met472. The last two start sites are accompanied by a deletion of aa 1241-1286, and a 62 aa substitution for the C-terminal 167 aa, respectively. Over aa 38-206, human Bcl9-2 is 94% aa identical to mouse Bcl9-2. Bcl9-2 has been identified as a phosphoprotein in several human cancer cell lines and mouse liver.
Product Datasheets
Citations for Human Bcl9-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
Authors: NA Watson, TN Cartwright, C Lawless, M Cámara-Don, O Sen, K Sako, T Hirota, H Kimura, JMG Higgins
Nat Commun, 2020-04-03;11(1):1684.
Species: Human
Sample Types: Cell Culture Lysates
Applications: ELISA Capture -
Pharmacological inhibition of ?-catenin/BCL9 interaction overcomes resistance to immune checkpoint blockades by modulating Treg cells
Authors: M Feng, JQ Jin, L Xia, T Xiao, S Mei, X Wang, X Huang, J Chen, M Liu, C Chen, S Rafi, AX Zhu, YX Feng, D Zhu
Sci Adv, 2019-05-08;5(5):eaau5240.
Species: N/A
Sample Types: Peptides
Applications: Immunoprecipitation -
Bcl9 and Pygo synergise downstream of Apc to effect intestinal neoplasia in FAP mouse models
Authors: J Mieszczane, LM van Tienen, AEK Ibrahim, DJ Winton, M Bienz
Nat Commun, 2019-02-13;10(1):724.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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