Human BDNF Antibody

Catalog #: MAB2481
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2481
MAB2481-SP

Detection of BDNF in U‑87 MG Human Cell Line by Flow Cytometry.

1 Image

All BDNF Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BDNF Antibody Summary

Species Reactivity

Human

Specificity

Detects human BDNF in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NT-3, rhNT-4, or rh beta -NGF is observed.

Source

Monoclonal Mouse IgG₁ Clone # 35909

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
His129-Arg247
Accession # P23560

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of BDNF antibody in U-87 MG Human Cell Line antibody by Flow Cytometry.

View Larger

Detection of BDNF in U‑87 MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB2481, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

References

Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
Snider, W.D. (1994) Cell 77:627.
Barbacid, M. (1994) J. Neurobiol. 25:1386.

Long Name

Brain-derived Neurotrophic Factor

Entrez Gene IDs

627 (Human); 12064 (Mouse); 24225 (Rat)

Alternate Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human BDNF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
Authors: Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C
J. Neuroimmunol., 2008-06-13;197(2):128-39.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr