Human beta -1 Adrenergic R/ADRB1 Antibody

Catalog #: MAB10119 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB10119-100
MAB10119-SP

Detection of Human beta ‑1 Adrenergic R/ADRB1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta -1 Adrenergic R/ADRB1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human beta ‑1 Adrenergic R/ADRB1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2203D

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human beta ‑1 Adrenergic R/ADRB1
Accession # P08588

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human beta ‑1 Adrenergic R/ADRB1 by Western Blot. Western blot shows lysates of human heart ventricle tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human beta -1 Adrenergic R/ADRB1 Monoclonal Antibody (Catalog # MAB10119) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for beta -1 Adrenergic R/ADRB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1 Adrenergic R/ADRB1

The beta-1 Adrenergic Receptor (ADRB1) is a 477 aminoacids long and seven-transmembrane domain protein that belongs to the G protein-coupled receptor (GPCR) 1 family. The adrenergic receptors, including ADRB1, are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. ADRB1 binds epinephrine and norepinephrine with approximately equal affinity. ADRB1 is localized at the plasma membrane. It is also found in the Golgi upon Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) overexpression.

Long Name

Beta-1 Adrenergic Receptor

Entrez Gene IDs

153 (Human); 11554 (Mouse); 24925 (Rat)

Alternate Names

ADRB1; ADRB1R; ADRB1RRHR; adrenergic, beta-1-, receptor; B1AR; beta-1 Adrenergic R; beta-1 adrenergic receptor; beta1 AdrenergicR; beta-1 AdrenergicR; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; BETA1AR