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Human beta-Catenin Antibody

Catalog #: MAB13291
7 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB13291-050
MAB13291-100
MAB13291-SP
beta ‑Catenin in SW480 Human Cell Line.
2 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta-Catenin Antibody Summary

Species Reactivity
Human
Specificity
Detects human  beta -Catenin in direct ELISAs.
Source
Monoclonal Mouse IgG3 Clone # 196618
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human beta -Catenin
aa 2-781
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human beta -Catenin
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry beta -Catenin antibody in SW480 Human Cell Line by Immunocytochemistry (ICC). View Larger

beta ‑Catenin in SW480 Human Cell Line. beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human beta -Catenin Monoclonal Antibody (Catalog # MAB13291) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry beta -Catenin antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

beta ‑Catenin in Human Pancreas. beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human beta -Catenin Monoclonal Antibody (Catalog # MAB13291) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Catenin

Beta-Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as as transcriptional co-activator in the nucleus and is also involved the canonical Wnt signal transduction pathways.

Entrez Gene IDs
1499 (Human); 12387 (Mouse); 84353 (Rat)
Alternate Names
bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923

Product Datasheets

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Citations for Human beta-Catenin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. A Potential Role of Semaphorin 3A during Orthodontic Tooth Movement
    Authors: S ?en, CJ Lux, R Erber
    International Journal of Molecular Sciences, 2021-08-02;22(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation
    Authors: GFA de Moraes, E Listik, GZ Justo, CM Vicente, L Toma
    BMC molecular and cell biology, 2021-05-04;22(1):26.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, IHC, Immunoprecipitation, Western Blot
  3. Tissue expression of ?-catenin and E- and N-cadherins in chronic hepatitis C and hepatocellular carcinoma
    Authors: A Kasprzak, K Rogacki, A Adamek, K Sterzy?ska, W Przybyszew, A Seraszek-J, C Helak-?apa, P Pyda
    Arch Med Sci, 2017-01-19;13(6):1269-1280.
    Species: Human
    Sample Types: Whole Tissue
  4. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015-07-15;10(7):e0128975.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells.
    Authors: Vicente C, Lima M, Nader H, Toma L
    J Exp Clin Cancer Res, 2015-03-14;34(0):25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC
  6. E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
    Authors: Moore RN, Cherry JF, Mathur V
    Stem Cells Dev., 2011-06-01;21(1):30-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Survivin in the human hair follicle.
    Authors: Botchkareva NV, Kahn M, Ahluwalia G, Shander D
    J. Invest. Dermatol., 2006-08-31;127(2):479-82.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Reviews for Human beta-Catenin Antibody

Average Rating: 4.8 (Based on 5 Reviews)

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Human beta-Catenin Antibody
By Anonymous on 11/02/2021
Application: ICC/IF Sample Tested: Bone marrow cells Species: Human
Immunocytochemistry/Immunofluorescence Human beta-Catenin Antibody MAB13291
Human beta -Catenin Antibody
By Anonymous on 11/03/2017
Application: ICC/IF Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human
Immunocytochemistry/Immunofluorescence Human beta -Catenin Antibody MAB13291
Human beta ‑Catenin Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Cell Lysates Species: Human

Specificity: SpecificSensitivity: SensitiveBuffer: 5% milk in TBSTDilution: 1/500

Human beta ‑Catenin Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: Human cell line Species: Human

Specificity: SpecificSensitivity: SensitiveBuffer: 1% BSA + 0.3% Triton X-100 in PBSDilution: 1/100

Human beta ‑Catenin Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: Human cell line Species: Human

Specificity: Reasonably specificSensitivity: Reasonably sensitiveBuffer: 1% BSA + 0.3% Triton X-100 in PBSDilution: 1/62.5