Human beta -NGF Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF256
Detection of Human beta-NGF by Flow Cytometry
3 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Human beta -NGF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -NGF in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant mouse beta ‑NGF is observed and less than 3% cross-reactivity with recombinant rat beta ‑NGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta -NGF (R&D Systems, Catalog # 256-GF)
Ser122-Ala241
Accession # CAA36832
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human beta -NGF (Catalog # 256-GF)

Human beta -NGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human beta ‑NGF Antibody (Catalog # MAB2561)

Capture Reagent: Human beta-NGF Antibody (Catalog # MAB256)

Standard: Recombinant Human beta-NGF Protein (Catalog # 256-GF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Human beta-NGF by Flow Cytometry View Larger

Detection of Human beta-NGF by Flow Cytometry NGF staining by flow cytometry. PBMC of (a) healthy controls (HC) and (b) synovial fluid mononuclear cells (SFMC) from spondyloarthritis (SpA), and (c) rheumatoid arthritis (RA) patients were first stained with surface markers (CD3 and CD14) and permeabilized in order to enable intracellular detection of nerve growth factor (NGF). The cells were analysed by flow cytometry after setting lymphocyte (left column) and monocyte (right column) gates according to forward scatter vs side scatter properties of the cells. Dot plots of one representative individual of each group are shown. Image collected and cropped by CiteAb from the following publication (https://arthritis-research.biomedcentral.com/articles/10.1186/ar2716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human beta-NGF by Flow Cytometry View Larger

Detection of Human beta-NGF by Flow Cytometry NGF staining by flow cytometry. PBMC of (a) healthy controls (HC) and (b) synovial fluid mononuclear cells (SFMC) from spondyloarthritis (SpA), and (c) rheumatoid arthritis (RA) patients were first stained with surface markers (CD3 and CD14) and permeabilized in order to enable intracellular detection of nerve growth factor (NGF). The cells were analysed by flow cytometry after setting lymphocyte (left column) and monocyte (right column) gates according to forward scatter vs side scatter properties of the cells. Dot plots of one representative individual of each group are shown. Image collected and cropped by CiteAb from the following publication (https://arthritis-research.biomedcentral.com/articles/10.1186/ar2716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human beta-NGF by Flow Cytometry View Larger

Detection of Human beta-NGF by Flow Cytometry NGF staining by flow cytometry. PBMC of (a) healthy controls (HC) and (b) synovial fluid mononuclear cells (SFMC) from spondyloarthritis (SpA), and (c) rheumatoid arthritis (RA) patients were first stained with surface markers (CD3 and CD14) and permeabilized in order to enable intracellular detection of nerve growth factor (NGF). The cells were analysed by flow cytometry after setting lymphocyte (left column) and monocyte (right column) gates according to forward scatter vs side scatter properties of the cells. Dot plots of one representative individual of each group are shown. Image collected and cropped by CiteAb from the following publication (https://arthritis-research.biomedcentral.com/articles/10.1186/ar2716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

Product Datasheets

You must select a language.

x

Citations for Human beta -NGF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
    Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
    PLoS ONE, 2017-01-09;12(1):e0168565.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  2. Inhibitory Effects of Polyacetylene Compounds from Panax ginseng on Neurotrophin Receptor-Mediated Hair Growth
    Authors: A Suzuki, D Matsuura, H Kanatani, S Yano, M Tsunakawa, S Matsuyama, H Shigemori
    Biol. Pharm. Bull., 2017-01-01;40(10):1784-1788.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  3. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  4. Nerve growth factor and receptor expression in rheumatoid arthritis and spondyloarthritis.
    Authors: Barthel C, Yeremenko N, Jacobs R
    Arthritis Res. Ther., 2009-06-02;11(3):R82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Nerve growth factor induces increased airway inflammation via a neuropeptide-dependent mechanism in a transgenic animal model of allergic airway inflammation.
    Authors: Quarcoo D, Schulte-Herbruggen O, Lommatzsch M, Schierhorn K, Hoyle GW, Renz H, Braun A
    Clin. Exp. Allergy, 2004-07-01;34(7):1146-51.
    Species: Transgenic Mouse
    Sample Types: BALF
    Applications: ELISA Development
  6. Nerve growth factor overexpression and autocrine loop in breast cancer cells.
    Authors: Dolle L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H
    Oncogene, 2003-08-28;22(36):5592-601.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human beta -NGF Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human beta -NGF Biotinylated Antibody and earn rewards!

Have you used Human beta -NGF Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review