Human Betacellulin/BTC Biotinylated Antibody Summary
Asp32-Tyr111
Accession # P35070
Applications
Human Betacellulin/BTC Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Betacellulin/BTC
Betacellulin (BTC) is a member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.
Product Datasheets
Citations for Human Betacellulin/BTC Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Locally advanced rectal cancer transcriptomic-based secretome analysis reveals novel biomarkers useful to identify patients according to neoadjuvant chemoradiotherapy response
Authors: LMD Canto, SS Cury, MC Barros-Fil, BEC Kupper, MDFS Begnami, C Scapulatem, RF Carvalho, FA Marchi, DA Olsen, JS Madsen, BM Havelund, S Aguiar, SR Rogatto
Sci Rep, 2019-06-18;9(1):8702.
Species: Human
Sample Types: Serum
Applications: ELISA Detection -
Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction
Authors: Tom J Parry
Eur. J. Pharmacol, 2016-12-16;796(0):76-89.
Species: Human
Sample Types: Recombinant Protein
Applications: Competitive Binding Assay -
Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke.
Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO
Neuropharmacology, 2010-08-04;59(7):640-9.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development -
Remission of diabetes by beta-cell regeneration in diabetic mice treated with a recombinant adenovirus expressing betacellulin.
Authors: Shin S, Li N, Kobayashi N, Yoon JW, Jun HS
Mol. Ther., 2008-03-18;16(5):854-61.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Hydrogen peroxide and endothelin-1 are novel activators of betacellulin ectodomain shedding.
Authors: Sanderson MP, Abbott CA, Tada H, Seno M, Dempsey PJ, Dunbar AJ
J. Cell. Biochem., 2006-10-01;99(2):609-23.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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