Home / BMPR-IA/ALK-3 / Human BMPR‑IA/ALK‑3 Biotinylated Antibody

Human BMPR‑IA/ALK‑3 Biotinylated Antibody

Catalog #: BAF820
5 Citations Datasheet / COA / SDS
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BAF820
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Citations (5)
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Human BMPR‑IA/ALK‑3 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMPR-IA in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) BMPR‑IA is observed and less than 5% cross-reactivity with rmBMPR-IB and recombinant human BMPR-II is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMPR-IA/ALK-3
Gln24-Arg152
Accession # P36894
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Antigen Affinity-purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BMPR-IA/ALK‑3 Fc Chimera (Catalog # 315-BR)
Flow Cytometry
2.5 µg/106 cells
PC‑3 human prostate cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMPR-IA/ALK-3

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IA (BMPR-IA), also known as activin receptor-like kinase (ALK)-3, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IA binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IA is ubiquitously expressed during embryogenesis. In adult tissues, BMPR-IA mRNA is also widely distributed with the highest expression levels found in skeletal muscle. The extracellular domain of BMPR-IA shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IA are highly conserved and share 98% sequence identity.

References
  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
  2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.
Long Name
Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3
Entrez Gene IDs
657 (Human); 12166 (Mouse)
Alternate Names
ACVRLK310q23del; ALK-3; ALK3EC 2.7.11.30; BMP type-1A receptor; BMPR1A; BMPR-1A; BMPRIA; BMPR-IA; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292 antigen; CD292; EC 2.7.11; Serine/threonine-protein kinase receptor R5; SKR5; type II-like kinase 3

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Citations for Human BMPR‑IA/ALK‑3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5
    Authors: LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust
    PLoS ONE, 2017-05-10;12(5):e0177188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma.
    Authors: Huse K, Bakkebo M, Walchli S, Oksvold M, Hilden V, Forfang L, Bredahl M, Liestol K, Alizadeh A, Smeland E, Myklebust J
    PLoS ONE, 2012-10-01;7(10):e46117.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: differential effects of BMP-6 and BMP-7.
    Authors: Huse K, Bakkebo M, Oksvold MP, Forfang L, Hilden VI, Stokke T, Smeland EB, Myklebust JH
    Eur. J. Immunol., 2011-10-18;41(11):3135-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Inhibitory effects and target genes of bone morphogenetic protein 6 in Jurkat TAg cells.
    Authors: Sivertsen EA, Huse K, Hystad ME, Kersten C, Smeland EB, Myklebust JH
    Eur. J. Immunol., 2007-10-01;37(10):2937-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3.
    Authors: Kersten C, Dosen G, Myklebust JH, Sivertsen EA, Hystad ME, Smeland EB, Rian E
    Exp. Hematol., 2006-01-01;34(1):72-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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