Human Bora Antibody

Catalog #: MAB6856 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6856
MAB6856-SP

1 Image

All Bora Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Bora Antibody Summary

Species Reactivity

Human

Specificity

Detects human Bora in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 694730

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Bora
Gly2-Ala180
Accession # Q6PGQ7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

Bora in MCF‑7 Human Cell Line. Bora was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Bora Monoclonal Antibody (Catalog # MAB6856) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bora

Bora (Aurora borealis; also C13orf34) is a 61 kDa member of the Bora family of proteins. It is ubiquitously expressed, and plays a key role in cell cycle progression. Plk1 (polo-like kinase-1) is a phosphorylase that is important to the cell during the G2/M transition and mitosis. Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on Thr210. Bora, Aurora-A and Plk1 all appear to form a complex during G2. Bora predisposes Plk1 to the actions of Aurora-A. Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora. This initiates BORA dissociation from Aurora-A with subsequent ubiquitination and degradation. Human Bora is 559 amino acids (aa) in length. It contains a Ser-rich region (aa 188-278) and at least eight utilized Ser phosphorylation sites. Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85 kDa. There is one potential alternative start site that lies 60 aa upstream of the standard start site, and a second splice variant the shows a 17 aa substitution for aa 1-87. Over aa 2-180, human Bora shares 84% aa identity with mouse Bora.

Long Name

Protein Aurora Borealis

Entrez Gene IDs

79866 (Human); 777441 (Mouse); 306102 (Rat)

Alternate Names

Aurora Borealis; Bora; C13orf34; chromosome 13 open reading frame 34; FLJ22624; hsBora; protein aurora borealis