Human BTN3A1/2/3 APC-conjugated Antibody

Catalog #: FAB7136A Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB7136A-025

Detection of BTN3A1/2/3 in Human Blood Lymphocytes by Flow Cytometry.

1 Image

All BTN3A1/2/3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BTN3A1/2/3 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 849203

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD277/BTN3A1
Met1-Gly254
Accession # O00481

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of BTN3A1/2/3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human BTN3A1/2/3 APC-conjugated Monoclonal Antibody (Catalog # FAB7136A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: BTN3A1/2/3

Butyrophilin BTN3A1, also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.

Long Name

Butyrophilin, Subfamily 3, Member A1/2/3

Alternate Names

BT3.1;BTF5;BTN3.1;BTN3A1;CD277; BTN3A1/2/3