Human BTN3A1/2/3 Antibody

Catalog #: AF7136 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7136
AF7136-SP

Detection of Human BTN3A1/2/3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BTN3A1/2/3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BTN3A2
Gln30-Trp248
Accession # P78410

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BTN3A1/2/3 by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BTN3A1/2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7136) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BTN3A1/2/3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN3A1/2/3

BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region (aa 30-248) with one V-type Ig-like domain (aa 30-134), and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. One possesses an alternative start site 43 aa upstream of the standard site, a second contains a five aa substitution for aa 1-28, and a third shows a 24 aa substitution for aa 307-334. A rodent counterpart to BTN3A2 has not been reported. Human BTN3A1 (Butyrophilin subfamily 3 member A1; also BTF5) over aa 30-248 shares 95.4% aa identity with human NTN3A2.

Long Name

Butyrophilin, Subfamily 3, Member A1/2/3

Alternate Names

BT3.1;BTF5;BTN3.1;BTN3A1;CD277; BTN3A1/2/3