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Human c-Myc PE-conjugated Antibody

Catalog #: IC3696P
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC3696P
Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry.
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Citations (4)
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Human c-Myc PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 9E10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C-terminal region peptide of human c-Myc
Ala408-Ala439
Accession # P01106
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of c-Myc antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc PE-conjugated Monoclonal Antibody (Catalog # IC3696P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max.  It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

 

Long Name
v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
Entrez Gene IDs
4609 (Human); 17869 (Mouse); 24577 (Rat)
Alternate Names
avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian)

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Citations for Human c-Myc PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
    Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
    J. Biol. Chem., 2020-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. "Inflammescent" CX3CR1+CD57+ CD8 T cells are generated and expanded by IL-15
    Authors: SR Morris, B Chen, JC Mudd, S Panigrahi, CL Shive, SF Sieg, CM Cameron, DA Zidar, NT Funderburg, SA Younes, B Rodriguez, S Gianella, MM Lederman, ML Freeman
    JCI Insight, 2020-06-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
  3. Glycosylation of Specific Notch EGF Repeats by O-Fut1 and Fringe Regulates Notch Signaling in Drosophila
    Authors: A Pandey, BM Harvey, MF Lopez, A Ito, RS Haltiwange, H Jafar-Neja
    Cell Rep, 2019-11-12;29(7):2054-2066.e6.
    Species: Drosophila melanogaster
    Sample Types: Whole Cell
    Applications: Flow Cytometry
  4. Abnormal Expression of c-Myc Oncogene in NK Cells in Patients with Cancer
    Authors: GK Zakiryanov, E Kustova, NT Urazalieva, ET Baimuchame, NN Nakisbekov, MR Shurin
    Int J Mol Sci, 2019-02-11;20(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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